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Literature summary extracted from
- Experimentally restrained molecular dynamics simulations for characterizing the open states of cytochrome P450cam (2011), Biochemistry, 50, 1664-1671.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.15.1 | expression of P450cam C334A in Escherichia coli NCM533 | Pseudomonas putida |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.14.15.1 | C334A | the mutation of P450cam increases the protein stability compared to the wild-type enzyme | Pseudomonas putida |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.15.1 | soluble | - |
Pseudomonas putida | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.15.1 | Fe2+ | heme protein | Pseudomonas putida |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.15.1 | 46000 | - |
1 * 46000 | Pseudomonas putida |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.15.1 | Pseudomonas putida | P00183 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.15.1 | recombinant P450cam C334A from Escherichia coli NCM533. Protein purification includes a protamine sulfate cut to precipitate nucleic acids, and an ammonium sulfate cut to isolate CYP101A1 | Pseudomonas putida |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.15.1 | monomer | 1 * 46000 | Pseudomonas putida |
| 1.14.15.1 | More | three-dimensional structure and dynamics of the open and closed states of CYP101A1, NMR analysis and molecular dynamics simulations, overview | Pseudomonas putida |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.15.1 | camphor monooxygenase | - |
Pseudomonas putida |
| 1.14.15.1 | CYP101A1 | - |
Pseudomonas putida |
| 1.14.15.1 | cytochrome p450cam | - |
Pseudomonas putida |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.15.1 | heme | - |
Pseudomonas putida | |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.14.15.1 | additional information | CYP101A1 is a soluble monomeric heme-containing camphor monooxygenase | Pseudomonas putida |
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Release 2023.1 (January 2023)
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