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Literature summary extracted from
- Kinetic analysis of 14-3-3-inhibited Arabidopsis thaliana nitrate reductase (2010), Biochemistry, 49, 8177-8186.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.7.1.1 | mutant S534A is expressed in Pichia pastoris strainKM71 | Arabidopsis thaliana |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.7.1.1 | S534A | the mutant is not inhibited by 14-3-3 proteins | Arabidopsis thaliana |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.7.1.1 | 14-3-3 protein isoform chi | noncompetitive inhibition with a preferential binding to the substrate-bound state of the enzyme | Arabidopsis thaliana | |
| 1.7.1.1 | 14-3-3 protein isoform epsilon | noncompetitive inhibition with a preferential binding to the substrate-bound state of the enzyme | Arabidopsis thaliana | |
| 1.7.1.1 | 14-3-3 protein isoform kappa | noncompetitive inhibition with a preferential binding to the substrate-bound state of the enzyme | Arabidopsis thaliana | |
| 1.7.1.1 | 14-3-3 protein isoform lambda | noncompetitive inhibition with a preferential binding to the substrate-bound state of the enzyme | Arabidopsis thaliana | |
| 1.7.1.1 | 14-3-3 protein isoform ni | noncompetitive inhibition with a preferential binding to the substrate-bound state of the enzyme | Arabidopsis thaliana | |
| 1.7.1.1 | 14-3-3 protein isoform omega | noncompetitive inhibition with a preferential binding to the substrate-bound state of the enzyme | Arabidopsis thaliana | |
| 1.7.1.1 | 14-3-3 protein isoform phi | noncompetitive inhibition with a preferential binding to the substrate-bound state of the enzyme | Arabidopsis thaliana | |
| 1.7.1.1 | 14-3-3 protein isoform theta | noncompetitive inhibition with a preferential binding to the substrate-bound state of the enzyme | Arabidopsis thaliana |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.7.1.1 | 0.197 | - |
nitrate | 22°C, pH not specified in the publication | Arabidopsis thaliana |  |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.7.1.1 | nitrate + NADH + H+ | Arabidopsis thaliana | - |
nitrite + NAD+ + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.7.1.1 | Arabidopsis thaliana | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 1.7.1.1 | phosphoprotein | - |
Arabidopsis thaliana |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.7.1.1 | SourceQ15 column chromatography and Ni-NTA column chromatography | Arabidopsis thaliana |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.7.1.1 | nitrate + NADH + H+ | - |
Arabidopsis thaliana | nitrite + NAD+ + H2O | - |
? | |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.7.1.1 | 33 | - |
nitrate | 22°C, pH not specified in the publication | Arabidopsis thaliana | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.7.1.1 | heme | - |
Arabidopsis thaliana | |
| 1.7.1.1 | NADH | - |
Arabidopsis thaliana | |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.7.1.1 | 0.00006 | - |
14-3-3 protein isoform omega | 22°C, pH not specified in the publication | Arabidopsis thaliana | |
| 1.7.1.1 | 0.00008 | - |
14-3-3 protein isoform lambda | 22°C, pH not specified in the publication | Arabidopsis thaliana | |
| 1.7.1.1 | 0.00014 | - |
14-3-3 protein isoform kappa | 22°C, pH not specified in the publication | Arabidopsis thaliana | |
| 1.7.1.1 | 0.00023 | - |
14-3-3 protein isoform ni | 22°C, pH not specified in the publication | Arabidopsis thaliana | |
| 1.7.1.1 | 0.00031 | - |
14-3-3 protein isoform phi | 22°C, pH not specified in the publication | Arabidopsis thaliana | |
| 1.7.1.1 | 0.00034 | - |
14-3-3 protein isoform chi | 22°C, pH not specified in the publication | Arabidopsis thaliana | |
| 1.7.1.1 | 0.00076 | - |
14-3-3 protein isoform theta | 22°C, pH not specified in the publication | Arabidopsis thaliana |
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