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Literature summary extracted from

  • Qiu, J.A.; Wilson, H.L.; Pushie, M.J.; Kisker, C.; George, G.N.; Rajagopalan, K.V.
    The structures of the C185S and C185A mutants of sulfite oxidase reveal rearrangement of the active site (2010), Biochemistry, 49, 3989-4000.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.2.1 expressed in Escherichia coli TP1000 cells Gallus gallus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.8.2.1 the C185S variant is crystallized by the hanging drop vapor diffusion method using 15% (v/v) 2-methyl-2,4-pentanediol, 2% PEG 4000 (w/v), and 100 mM sodium acetate (pH 5.0) and in the presence of sulfite using 2% PEG 4000 (w/v), 10% MPD (v/v), 100 mM sodium acetate (pH 5.0) and 0.5 mM sodium sulfite. The C185A variant is crystallized by the sitting drop vapor diffusion method, using 15% (w/v) PEG 4000, 150 mM (NH4)2SO4, and 100 mM MES (pH 6.0) Gallus gallus

Protein Variants

EC Number Protein Variants Comment Organism
1.8.2.1 C185A the active site of the mutant is essentially catalytically inactive with ferricyctochrome c or ferricyanide as electron acceptor Gallus gallus
1.8.2.1 C185S the active site of the mutant is essentially catalytically inactive with ferricyctochrome c or ferricyanide as electron acceptor Gallus gallus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.2.1 sulfite + ferricytochrome c + H2O Gallus gallus ferricytochrome c is the physiological electron acceptor sulfate + ferrocytochrome c + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.2.1 Gallus gallus P07850
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.2.1 phenyl-Sepharose column chromatography and Superdex-200 gel filtration Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.2.1 liver
-
 Gallus gallus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.2.1 sulfite + ferricyanide + H2O
-
 Gallus gallus sulfate + ferrocyanide + H+
-
 ?
1.8.2.1 sulfite + ferricytochrome c + H2O ferricytochrome c is the physiological electron acceptor Gallus gallus sulfate + ferrocytochrome c + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.2.1 sulfate oxidase
-
 Gallus gallus

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.2.1 heme
-
 Gallus gallus
1.8.2.1 molybdopterin
-
 Gallus gallus