BRENDA - Enzyme Database

The membrane-bound quinohemoprotein alcohol dehydrogenase from Gluconacetobacter diazotrophicus PAL5 carries a [2Fe-2S] cluster

Gomez-Manzo, S.; Solano-Peralta, A.; Saucedo-Vazquez, J.P.; Escamilla-Marvan, J.E.; Kroneck, P.M.; Sosa-Torres, M.E.; Biochemistry 49, 2409-2415 (2010)

Data extracted from this reference:

Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.5.5
membrane
-
Gluconacetobacter diazotrophicus
16020
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.5.5
Ca2+
the enzyme contains one calcium ion which is required for cofactor binding and stabilization of the pyrroloquinoline quinone semiquinone radical
Gluconacetobacter diazotrophicus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.5.5
44000
-
1 * 71000 + 1 * 44000, SDS-PAGE
Gluconacetobacter diazotrophicus
1.1.5.5
71000
-
1 * 71000 + 1 * 44000, SDS-PAGE
Gluconacetobacter diazotrophicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.5.5
Gluconacetobacter diazotrophicus
-
-
-
1.1.5.5
Gluconacetobacter diazotrophicus PAL5 (ATCC 49037)
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.5.5
-
Gluconacetobacter diazotrophicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.5.5
ethanol + ferricyanide
-
711227
Gluconacetobacter diazotrophicus
acetaldehyde + ferrocyanide
-
-
-
?
1.1.5.5
ethanol + ferricyanide
-
711227
Gluconacetobacter diazotrophicus PAL5 (ATCC 49037)
acetaldehyde + ferrocyanide
-
-
-
?
1.1.5.5
ethanol + phenazine methosulfate + 2,6-dichlorophenolindophenol
-
711227
Gluconacetobacter diazotrophicus
?
-
-
-
?
1.1.5.5
ethanol + phenazine methosulfate + 2,6-dichlorophenolindophenol
-
711227
Gluconacetobacter diazotrophicus PAL5 (ATCC 49037)
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.5.5
heterodimer
1 * 71000 + 1 * 44000, SDS-PAGE
Gluconacetobacter diazotrophicus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.5.5
cytochrome c
ADH contains 4 c-type cytochromes
Gluconacetobacter diazotrophicus
1.1.5.5
heme
ADH is a typical quinohemoprotein
Gluconacetobacter diazotrophicus
1.1.5.5
pyrroloquinoline quinone
ADH is a typical quinohemoprotein with one pyrroloquinoline quinone
Gluconacetobacter diazotrophicus
1.1.5.5
[2Fe-2S]-center
ADH contains 5.9 Fe2+ and 2.06 acid-labile sulfurs per heterodimer
Gluconacetobacter diazotrophicus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.5.5
cytochrome c
ADH contains 4 c-type cytochromes
Gluconacetobacter diazotrophicus
1.1.5.5
heme
ADH is a typical quinohemoprotein
Gluconacetobacter diazotrophicus
1.1.5.5
pyrroloquinoline quinone
ADH is a typical quinohemoprotein with one pyrroloquinoline quinone
Gluconacetobacter diazotrophicus
1.1.5.5
[2Fe-2S]-center
ADH contains 5.9 Fe2+ and 2.06 acid-labile sulfurs per heterodimer
Gluconacetobacter diazotrophicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.5.5
membrane
-
Gluconacetobacter diazotrophicus
16020
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.5.5
Ca2+
the enzyme contains one calcium ion which is required for cofactor binding and stabilization of the pyrroloquinoline quinone semiquinone radical
Gluconacetobacter diazotrophicus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.5.5
44000
-
1 * 71000 + 1 * 44000, SDS-PAGE
Gluconacetobacter diazotrophicus
1.1.5.5
71000
-
1 * 71000 + 1 * 44000, SDS-PAGE
Gluconacetobacter diazotrophicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.5.5
-
Gluconacetobacter diazotrophicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.5.5
ethanol + ferricyanide
-
711227
Gluconacetobacter diazotrophicus
acetaldehyde + ferrocyanide
-
-
-
?
1.1.5.5
ethanol + ferricyanide
-
711227
Gluconacetobacter diazotrophicus PAL5 (ATCC 49037)
acetaldehyde + ferrocyanide
-
-
-
?
1.1.5.5
ethanol + phenazine methosulfate + 2,6-dichlorophenolindophenol
-
711227
Gluconacetobacter diazotrophicus
?
-
-
-
?
1.1.5.5
ethanol + phenazine methosulfate + 2,6-dichlorophenolindophenol
-
711227
Gluconacetobacter diazotrophicus PAL5 (ATCC 49037)
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.5.5
heterodimer
1 * 71000 + 1 * 44000, SDS-PAGE
Gluconacetobacter diazotrophicus