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Literature summary extracted from

  • Christensen, Q.H.; Cronan, J.E.
    Lipoic acid synthesis: a new family of octanoyltransferases generally annotated as lipoate protein ligases (2010), Biochemistry, 49, 10024-10036.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.181 expression in Escherichia coli Bacillus subtilis

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.181 C150A loss of the overall catalytic activity and an inability to form the acyl-enzyme intermediate Bacillus subtilis
2.3.1.181 C150S loss of the overall catalytic activity and an inability to form the acyl-enzyme intermediate Bacillus subtilis
2.3.1.181 K165A mutant has weakened catalytic ability Bacillus subtilis
2.3.1.181 K165R mutant remains active Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.181 14000
-
 gel filtration Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.181 octanoyl-[acyl-carrier protein] + a protein Bacillus subtilis
-
 a protein N6-(octanoyl)lysine + an [acyl-carrier protein]
-
 ?
2.8.1.8 protein N6-(octanoyl)lysine + sulfur + S-adenosyl-L-methionine Bacillus subtilis
-
 protein N6-(lipoyl)lysine + L-methionine + 5'-deoxyadenosine
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.181 Bacillus subtilis
-
-
-
2.8.1.8 Bacillus subtilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.181
-
 Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.181 octanoyl-[acyl-carrier protein] + a protein
-
 Bacillus subtilis a protein N6-(octanoyl)lysine + an [acyl-carrier protein]
-
 ?
2.3.1.181 octanoyl-[acyl-carrier protein] + a protein the transfer of the octanoyl group from octanoyl-ACP to Bacillus subtilis GcvH occurs in a LipM-dependent manner. The LipM reaction proceeds through a thioester-linked acyl enzyme intermediate. The LipM active site nucleophile is identified as C150 Bacillus subtilis a protein N6-(octanoyl)lysine + an [acyl-carrier protein]
-
 ?
2.8.1.8 protein N6-(octanoyl)lysine + sulfur + S-adenosyl-L-methionine
-
 Bacillus subtilis protein N6-(lipoyl)lysine + L-methionine + 5'-deoxyadenosine
-
 ir

Subunits

EC Number Subunits Comment Organism
2.3.1.181 monomer 1 * 14000, SDS-PAGE Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
2.3.1.181 LipM
-
 Bacillus subtilis
2.3.1.181 octanoyltransferase LipM
-
 Bacillus subtilis
2.8.1.8 LipA
-
 Bacillus subtilis
2.8.1.8 lipoate synthase
-
 Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.181 37
-
 assay at Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.181 7
-
 assay at Bacillus subtilis

Cofactor

EC Number Cofactor Comment Organism Structure
2.8.1.8 S-adenosyl-L-methionine
-
 Bacillus subtilis