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Literature summary extracted from

  • Baurin, S.; Vercheval, L.; Bouillenne, F.; Falzone, C.; Brans, A.; Jacquamet, L.; Ferrer, J.L.; Sauvage, E.; Dehareng, D.; Frere, J.M.; Charlier, P.; Galleni, M.; Kerff, F.
    Critical role of tryptophan 154 for the activity and stability of class D beta-lactamases (2009), Biochemistry, 48, 11252-11263.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.2.6 hanging drop vapor diffusion method, mutant enzyme W154A, mutant enzyme W154G, mutant enzyme W154H plasmid pGEM-T-easy

Protein Variants

EC Number Protein Variants Comment Organism
3.5.2.6 W154F noncarboxylated enzymes which displays poor catalytic efficiencies and reduced stability when compared to the wild-type OXA-10 plasmid pGEM-T-easy
3.5.2.6 W154G noncarboxylated enzymes which displays poor catalytic efficiencies and reduced stability when compared to the wild-type OXA-10 plasmid pGEM-T-easy
3.5.2.6 W154H noncarboxylated enzymes which displays poor catalytic efficiencies and reduced stability when compared to the wild-type OXA-10 plasmid pGEM-T-easy

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.6 0.004
-
 cephalothin mutant enzyme W154H, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.005
-
 cephalothin mutant enzyme W154H, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.005
-
 cephalothin wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.007
-
 cephalothin wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.01
-
 benzylpenicillin mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.01
-
 nitrocefin mutant enzyme W154H, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.01
-
 nitrocefin wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.013
-
 nitrocefin mutant enzyme W154H, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.017
-
 benzylpenicillin mutant enzyme W154H, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.02
-
 Oxacillin mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.02
-
 benzylpenicillin wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.025
-
 cephalothin mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.025
-
 nitrocefin wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.03
-
 ampicillin mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.03
-
 Cloxacillin mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.035
-
 nitrocefin mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.035
-
 ampicillin wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.04
-
 cephalothin mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.04
-
 ampicillin mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.05
-
 nitrocefin mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.05
-
 cephaloridine mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.055
-
 benzylpenicillin wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.06
-
 ampicillin mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.06
-
 Oxacillin mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.06
-
 benzylpenicillin mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.06
-
 cephaloridine mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.06
-
 nitrocefin mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.065
-
 cephaloridine mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.07
-
 benzylpenicillin mutant enzyme W154H, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.1
-
 benzylpenicillin mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.1
-
 nitrocefin mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.1
-
 Cloxacillin mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.1
-
 Oxacillin mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.1
-
 Oxacillin wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.11
-
 nitrocefin mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.11
-
 Cloxacillin wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.125
-
 ampicillin mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.125
-
 Oxacillin mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.13
-
 Oxacillin mutant enzyme W154H, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.14
-
 Cloxacillin mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.15
-
 benzylpenicillin mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.15
-
 ampicillin mutant enzyme W154H, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.18
-
 ampicillin wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.2
-
 Oxacillin mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.2
-
 Cloxacillin mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.2
-
 Oxacillin wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.21
-
 cephalothin mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.21
-
 cephalothin mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.22
-
 Cloxacillin mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.23
-
 ampicillin mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.25
-
 ampicillin mutant enzyme W154H, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.25
-
 Cloxacillin wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.29
-
 cephaloridine mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.4
-
 cephaloridine wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.45
-
 cephaloridine mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.45
-
 cephalothin mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.6
-
 benzylpenicillin mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.65
-
 Oxacillin mutant enzyme W154H, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 2
-
 cephaloridine wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.6 plasmid pGEM-T-easy
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.6
-
 plasmid pGEM-T-easy

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.6 ampicillin + H2O
-
 plasmid pGEM-T-easy (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 benzylpenicillin + H2O
-
 plasmid pGEM-T-easy (2R,4S)-2-[(R)-carboxy[(phenylacetyl)amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 cephaloridine + H2O
-
 plasmid pGEM-T-easy (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
3.5.2.6 cephalothin + H2O
-
 plasmid pGEM-T-easy (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cloxacillin + H2O
-
 plasmid pGEM-T-easy (2R,4S)-2-[(R)-carboxy([[3-(2-chlorophenyl)-5-methyl-1,2-oxazol-4-yl]carbonyl]amino)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 nitrocefin + H2O
-
 plasmid pGEM-T-easy (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 oxacillin + H2O
-
 plasmid pGEM-T-easy (2R,4S)-2-[(R)-carboxy[[(5-methyl-3-phenyl-1,2-oxazol-4-yl)carbonyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.2.6 dimer
-
 plasmid pGEM-T-easy

Synonyms

EC Number Synonyms Comment Organism
3.5.2.6 beta-lactamase OXA-10
-
 plasmid pGEM-T-easy

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.2.6 39.9
-
 t1/2 for mutant enzyme W154A, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 41.9
-
 t1/2 for mutant enzyme W154G, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 48.8
-
 t1/2 for mutant enzyme W154F, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 50.7
-
 t1/2 for mutant enzyme W154G, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 53.5
-
 t1/2 for mutant enzyme W154F, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 55.9
-
 t1/2 for wild-type enzyme, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 56.7
-
 t1/2 for mutant enzyme W154A, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 60
-
 t1/2 for wild-type enzyme, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.2.6 0.002
-
 cephaloridine mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.0023
-
 cephalothin mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.003
-
 cephalothin mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.003
-
 cephalothin mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.0036
-
 cephaloridine mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.004
-
 cephaloridine mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.012
-
 cephalothin mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.0126
-
 ampicillin mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.015
-
 cephaloridine mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.04
-
 benzylpenicillin mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.07
-
 ampicillin mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.08
-
 benzylpenicillin mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.1
-
 cephalothin mutant enzyme W154H, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.13
-
 benzylpenicillin mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.132
-
 ampicillin mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.15
-
 cephalothin mutant enzyme W154H, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.2
-
 Cloxacillin mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.25
-
 Oxacillin mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.6
-
 nitrocefin mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.7
-
 benzylpenicillin mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 1
-
 nitrocefin mutant enzyme W154H, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 1.3
-
 cephaloridine mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 1.4
-
 ampicillin mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 1.5
-
 benzylpenicillin mutant enzyme W154H, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 1.8
-
 Cloxacillin mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 2
-
 nitrocefin mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 2
-
 Oxacillin mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 2
-
 cephalothin wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 2.4
-
 ampicillin mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 2.5
-
 benzylpenicillin mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 2.5
-
 Cloxacillin mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 2.5
-
 nitrocefin mutant enzyme W154H, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 2.5
-
 cephalothin wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 3
-
 ampicillin mutant enzyme W154H, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 3.5
-
 Oxacillin mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 3.5
-
 Cloxacillin mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 4
-
 Cloxacillin mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 4
-
 nitrocefin mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 4
-
 Oxacillin mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 4
-
 benzylpenicillin mutant enzyme W154H, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 4.5
-
 Oxacillin mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 6
-
 nitrocefin mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 8
-
 cephalothin mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 8
-
 nitrocefin mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 18
-
 ampicillin mutant enzyme W154H, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 35
-
 Oxacillin mutant enzyme W154H, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 35
-
 cephaloridine wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 70
-
 cephaloridine wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 85
-
 benzylpenicillin wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 90
-
 Oxacillin mutant enzyme W154H, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 120
-
 benzylpenicillin wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 120
-
 Cloxacillin wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 160
-
 ampicillin wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 220
-
 ampicillin wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 300
-
 Oxacillin wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 500
-
 Cloxacillin wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 600
-
 Oxacillin wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 1300
-
 nitrocefin wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 1700
-
 nitrocefin wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.5.2.6 plasmid pGEM-T-easy OXA-10 wild-type enzyme
-
 6.6
3.5.2.6 plasmid pGEM-T-easy W154 OXA-10 mutant
-
 7.7

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.2.6 0.014
-
 cephalothin mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.03
-
 cephaloridine mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.056
-
 ampicillin mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.067
-
 benzylpenicillin mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.25
-
 cephalothin mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.3
-
 nitrocefin mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 0.53
-
 benzylpenicillin mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 1.15
-
 ampicillin mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 2
-
 Cloxacillin mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 3.3
-
 ampicillin mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 4.2
-
 Oxacillin mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 5
-
 cephalothin mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 5.5
-
 nitrocefin mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 10
-
 cephaloridine mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 12
-
 ampicillin mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 12
-
 Cloxacillin mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 12
-
 cephaloridine mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 13
-
 Cloxacillin mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 13
-
 benzylpenicillin mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 13
-
 ampicillin mutant enzyme W154H, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 14
-
 cephalothin mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 16
-
 cephaloridine mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 16
-
 Oxacillin mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 17.5
-
 Oxacillin mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 18
-
 Cloxacillin mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 18
-
 cephaloridine wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 19
-
 ampicillin mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 20
-
 cephalothin mutant enzyme W154H, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 23
-
 cephaloridine mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 25
-
 benzylpenicillin mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 30
-
 nitrocefin mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 35
-
 cephalothin mutant enzyme W154H, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 40
-
 nitrocefin mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 40
-
 Oxacillin mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 47
-
 Cloxacillin mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 53
-
 Oxacillin mutant enzyme W154H, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 57
-
 benzylpenicillin mutant enzyme W154H, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 80
-
 benzylpenicillin mutant enzyme W154H, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 100
-
 nitrocefin mutant enzyme W154H, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 120
-
 ampicillin mutant enzyme W154H, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 130
-
 benzylpenicillin mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 130
-
 Oxacillin mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 130
-
 nitrocefin mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 180
-
 cephaloridine wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 190
-
 nitrocefin mutant enzyme W154H, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 320
-
 cephalothin mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 380
-
 cephalothin wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 400
-
 cephalothin wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 700
-
 Oxacillin mutant enzyme W154H, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 900
-
 ampicillin wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 1000
-
 Cloxacillin wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 1500
-
 benzylpenicillin wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 2000
-
 Cloxacillin wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 3000
-
 Oxacillin wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 3000
-
 Oxacillin wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 6000
-
 benzylpenicillin wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 6200
-
 ampicillin wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 52000
-
 nitrocefin wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate plasmid pGEM-T-easy
3.5.2.6 170000
-
 nitrocefin wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate plasmid pGEM-T-easy