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Literature summary extracted from

  • Matsunaga, I.; Yamada, A.; Lee, D.S.; Obayashi, E.; Fujiwara, N.; Kobayashi, K.; Ogura, H.; Shiro, Y.
    Enzymatic reaction of hydrogen peroxide-dependent peroxygenase cytochrome P450s: kinetic deuterium isotope effects and analyses by resonance Raman spectroscopy (2002), Biochemistry, 41, 1886-1892.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.2.4 expressed in Escherichia coli Sphingomonas paucimobilis
1.11.2.4 expressed in Escherichia coli Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.2.4 0.041
-
 myristic acid pH and temperature not specified in the publication Sphingomonas paucimobilis
1.11.2.4 0.058
-
 myristic acid pH and temperature not specified in the publication Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.11.2.4 additional information Sphingomonas paucimobilis P450BSbeta produces alpha- and beta-hydroxylated products at 33 and 67%, respectively ?
-
 ?
1.11.2.4 additional information Bacillus subtilis P450BSbeta produces alpha- and beta-hydroxylated products at 33 and 67%, respectively ?
-
 ?
1.11.2.4 additional information Sphingomonas paucimobilis P450SPalpha produces the alpha-hydroxylated products at 100% ?
-
 ?
1.11.2.4 additional information Bacillus subtilis P450SPalpha produces the alpha-hydroxylated products at 100% ?
-
 ?
1.11.2.4 myristic acid + H2O2 Sphingomonas paucimobilis
-
 ?
-
 ?
1.11.2.4 myristic acid + H2O2 Bacillus subtilis
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.11.2.4 Bacillus subtilis O31440
-
-
1.11.2.4 Sphingomonas paucimobilis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.2.4 2-hydroxylauric acid + H2O2
-
 Sphingomonas paucimobilis ?
-
 ?
1.11.2.4 2-hydroxylauric acid + H2O2
-
 Bacillus subtilis ?
-
 ?
1.11.2.4 additional information P450BSbeta produces alpha- and beta-hydroxylated products at 33 and 67%, respectively Sphingomonas paucimobilis ?
-
 ?
1.11.2.4 additional information P450BSbeta produces alpha- and beta-hydroxylated products at 33 and 67%, respectively Bacillus subtilis ?
-
 ?
1.11.2.4 additional information P450SPalpha produces the alpha-hydroxylated products at 100% Sphingomonas paucimobilis ?
-
 ?
1.11.2.4 additional information P450SPalpha produces the alpha-hydroxylated products at 100% Bacillus subtilis ?
-
 ?
1.11.2.4 myristic acid + H2O2
-
 Sphingomonas paucimobilis ?
-
 ?
1.11.2.4 myristic acid + H2O2
-
 Bacillus subtilis ?
-
 ?
1.11.2.4 myristic acid + H2O2
-
 Sphingomonas paucimobilis 2-hydroxy-myristic acid
-
 ?
1.11.2.4 myristic acid + H2O2
-
 Bacillus subtilis 2-hydroxy-myristic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.11.2.4 CYP152A1
-
 Bacillus subtilis
1.11.2.4 CYP152B1
-
 Sphingomonas paucimobilis
1.11.2.4 hydrogen peroxide-dependent peroxygenase cytochrome P450
-
 Sphingomonas paucimobilis
1.11.2.4 hydrogen peroxide-dependent peroxygenase cytochrome P450
-
 Bacillus subtilis
1.11.2.4 P450BSbeta
-
 Bacillus subtilis
1.11.2.4 P450SPalpha
-
 Sphingomonas paucimobilis