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Literature summary extracted from

  • Bansal, S.; Gnaneswari, D.; Mishra, P.; Kundu, B.
    Structural stability and functional analysis of L-asparaginase from Pyrococcus furiosus (2010), Biochemistry (Moscow), 75, 375-381.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.1 cloned and expressed in Escherichia coli Pyrococcus furiosus

General Stability

EC Number General Stability Organism
3.5.1.1 in the presence of guanidine hydrochloride (GdnCl) the curves follow a sigmoidal pattern with unfolding beginning at 3.25 M guanidine hydrochloride and complete unfolding achieved at 4.75 M guanidine hydrochloride. The enzymatic activity of urea-containing protein samples remains unchanged. In contrast, the guanidine hydrochloride-incubated samples show a significantly lower activity even with 1 M guanidine hydrochloride. Pyrococcus furiosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.1 L-asparagine substrate inhibition above 20 mM L-asparagine Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.1 37000
-
 2 * 37000, SDS-PAGE, 2 * 37860, mass spectrometry Pyrococcus furiosus
3.5.1.1 37860
-
 2 * 37000, SDS-PAGE, 2 * 37860, mass spectrometry Pyrococcus furiosus
3.5.1.1 75450
-
 gel filtration, Superdex-200 column, single peak Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.1 Pyrococcus furiosus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.1 to homogeneity Pyrococcus furiosus

Subunits

EC Number Subunits Comment Organism
3.5.1.1 dimer 2 * 37000, SDS-PAGE, 2 * 37860, mass spectrometry Pyrococcus furiosus

Synonyms

EC Number Synonyms Comment Organism
3.5.1.1 L-asparaginase
-
 Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.1 80
-
 assay at Pyrococcus furiosus
3.5.1.1 85
-
 about 85°C, activity increases progressively with increase in temperature up to and beyond 85°C Pyrococcus furiosus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.1 55 85 enzyme incubation for different time periods monitored over a range of 55-85°C shows linear kinetics. From the plot the activation energy is found to be 42.5 kJ/mol*K Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.1 9
-
-
 Pyrococcus furiosus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.1 6 10.5 an increase in enzyme activity is observed with increase in pH up to a maximum at pH 9 followed by a sharp fall Pyrococcus furiosus