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Literature summary extracted from

  • Pakhadnia, Y.G.; Malinouski, N.I.; Lapko, A.G.
    Purification and characteristics of an enzyme with both bilirubin oxidase and laccase activities from mycelium of the basidiomycete Pleurotus ostreatus (2009), Biochemistry (Moscow), 74, 1027-1034.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 0.02
-
syringaldazine isoform POXC, pH 6.0, 25°C Pleurotus ostreatus
1.10.3.2 0.036
-
syringaldazine isoform POXA3a, pH 6.0, 25°C Pleurotus ostreatus
1.10.3.2 0.07
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) isoform POXA3a, pH 3.6, 25°C Pleurotus ostreatus
1.10.3.2 0.074
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) isoform POXA3b, pH 3.6, 25°C Pleurotus ostreatus
1.10.3.2 0.079
-
syringaldazine isoform POXA3b, pH 6.0, 25°C Pleurotus ostreatus
1.10.3.2 0.09
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) isoform POXA1w, pH 3.0, 25°C Pleurotus ostreatus
1.10.3.2 0.12
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) isoform POXA2, pH 3.0, 25°C Pleurotus ostreatus
1.10.3.2 0.13
-
syringaldazine isoform POXA1w, pH 6.0, 25°C Pleurotus ostreatus
1.10.3.2 0.14
-
syringaldazine isoform POXA2, pH 6.0, 25°C Pleurotus ostreatus
1.10.3.2 0.22
-
syringaldazine isoform POXA1b, pH 6.0, 25°C Pleurotus ostreatus
1.10.3.2 0.23
-
2,6-dimethoxyphenol isoform POXC, pH 3.0-5.0, 25°C Pleurotus ostreatus
1.10.3.2 0.26
-
2,6-dimethoxyphenol isoform POXA1b, pH 4.0-5.0, 25°C Pleurotus ostreatus
1.10.3.2 0.28
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) isoform POXC, pH 3.0, 25°C Pleurotus ostreatus
1.10.3.2 0.37
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) isoform POXA1b, pH 3.0, 25°C Pleurotus ostreatus
1.10.3.2 0.74
-
2,6-dimethoxyphenol isoform POXA2, pH 6.5, 25°C Pleurotus ostreatus
1.10.3.2 1.2
-
guaiacol isoform POXC, pH 6.0, 25°C Pleurotus ostreatus
1.10.3.2 2.1
-
2,6-dimethoxyphenol isoform POXA1w, pH 3.0-5.0, 25°C Pleurotus ostreatus
1.10.3.2 3.1
-
guaiacol isoform POXA2, pH 6.0, 25°C Pleurotus ostreatus
1.10.3.2 8.8
-
2,6-dimethoxyphenol isoform POXA3b, pH 5.5, 25°C Pleurotus ostreatus
1.10.3.2 14
-
2,6-dimethoxyphenol isoform POXA3a, pH 5.5, 25°C Pleurotus ostreatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 59000
-
1 * 59000, isoform POXC, SDS-PAGE Pleurotus ostreatus
1.10.3.2 61000
-
1 * 61000, isoform POXA1w, SDS-PAGE Pleurotus ostreatus
1.10.3.2 62000
-
1 * 62000, isoform POXA1b, SDS-PAGE Pleurotus ostreatus
1.10.3.2 67000
-
1 * 67000, isoform POXA2, SDS-PAGE Pleurotus ostreatus

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Pleurotus ostreatus
-
-
-
1.10.3.2 Pleurotus ostreatus 43 BIM F-306D
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2 DEAE-cellulose column chromatography, ConA-Sepharose column chromatography, and Toyopearl HW-55 column chromatography Pleurotus ostreatus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.2 mycelium
-
Pleurotus ostreatus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.2 19.1
-
crude enzyme, using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate, at pH 3.0 and 50°C Pleurotus ostreatus
1.10.3.2 261
-
purified enzyme, using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate, at pH 3.0 and 50°C Pleurotus ostreatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
Pleurotus ostreatus ?
-
?
1.10.3.2 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
Pleurotus ostreatus 43 BIM F-306D ?
-
?
1.10.3.2 2,6-dimethoxyphenol + O2
-
Pleurotus ostreatus ?
-
?
1.10.3.2 2,6-dimethoxyphenol + O2
-
Pleurotus ostreatus 43 BIM F-306D ?
-
?
1.10.3.2 guaiacol + O2
-
Pleurotus ostreatus ?
-
?
1.10.3.2 guaiacol + O2
-
Pleurotus ostreatus 43 BIM F-306D ?
-
?
1.10.3.2 additional information the isolated enzyme from Pleurotus ostreatus mycelium has both bilirubin oxidase and laccase activities Pleurotus ostreatus ?
-
?
1.10.3.2 additional information the isolated enzyme from Pleurotus ostreatus mycelium has both bilirubin oxidase and laccase activities Pleurotus ostreatus 43 BIM F-306D ?
-
?
1.10.3.2 syringaldazine + O2
-
Pleurotus ostreatus ?
-
?
1.10.3.2 syringaldazine + O2
-
Pleurotus ostreatus 43 BIM F-306D ?
-
?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 monomer 1 * 59000, isoform POXC, SDS-PAGE Pleurotus ostreatus
1.10.3.2 monomer 1 * 61000, isoform POXA1w, SDS-PAGE Pleurotus ostreatus
1.10.3.2 monomer 1 * 62000, isoform POXA1b, SDS-PAGE Pleurotus ostreatus
1.10.3.2 monomer 1 * 67000, isoform POXA2, SDS-PAGE Pleurotus ostreatus
1.10.3.2 monomer 1 * 83000-85000, isoforms POXA3a and POXA3b, SDS-PAGE Pleurotus ostreatus

Synonyms

EC Number Synonyms Comment Organism
1.10.3.2 p-diphenol:oxygen oxidoreductase
-
Pleurotus ostreatus
1.10.3.2 POXA1b isoform Pleurotus ostreatus
1.10.3.2 POXA1w isoform Pleurotus ostreatus
1.10.3.2 POXA2 isoform Pleurotus ostreatus
1.10.3.2 POXA3a isoform Pleurotus ostreatus
1.10.3.2 POXA3b isoform Pleurotus ostreatus
1.10.3.2 POXC isoform Pleurotus ostreatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 20 50 isoform POXA1b Pleurotus ostreatus
1.10.3.2 25 35 isoform POXA2 Pleurotus ostreatus
1.10.3.2 35
-
isoforms POXA3a and POXA3b Pleurotus ostreatus
1.10.3.2 45 65 isoform POXA1w Pleurotus ostreatus
1.10.3.2 50 60 isoform POXC Pleurotus ostreatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 40 60 isoform POXA1b shows a inactivation half-period of 3 h at 60°C, isoform POXA1w shows a inactivation half-period of 3.3 h at 60°C, isoform POXA2 shows a inactivation half-period of 0.2 h at 60°C, isoform POXA3a shows a inactivation half-period of 6 h at 40°C, isoform POXA3b shows a inactivation half-period of 14 h at 40°C, isoform POXC shows a inactivation half-period of 30 min at 40°C Pleurotus ostreatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 3 6.5 using 2,6-dimethoxyphenol as substrate Pleurotus ostreatus
1.10.3.2 3 3.6 using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate Pleurotus ostreatus
1.10.3.2 6 7 using syringaldazine or guaiacol as substrate Pleurotus ostreatus