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Literature summary extracted from

  • Morrone, D.; Chen, X.; Coates, R.M.; Peters, R.J.
    Characterization of the kaurene oxidase CYP701A3, a multifunctional cytochrome P450 from gibberellin biosynthesis (2010), Biochem. J., 431, 337-344.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.86 CYP701A3, functional expression in Escherichia coli using a fully codon-optimized construct, along with additional N-terminal deletion and modification Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
1.14.14.86 additional information usage of a fully codon-optimized construct, along with additional N-terminal deletion and modification, for functional recombinant expression in Escherichia coli, recombinant engineered enzyme is used to carry out 18O2 labelling studies with ent-kaurene, and the intermediates ent-kaurenol and ent-kaurenal, to investigate the multifunctional reaction sequence, overview Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.14.86 (1E)-1-[4-(4-butyl-1H-1,2,3-triazol-1-yl)phenyl]-4,4-dimethyl-2-(1H-1,2,4-triazol-1-yl)pent-1-en-3-ol
-
 Arabidopsis thaliana
1.14.14.86 (1E,3S)-1-(4-chlorophenyl)-4,4-dimethyl-2-(1H-1,2,4-triazol-1-yl)pent-1-en-3-ol i.e. uniconazole Arabidopsis thaliana
1.14.14.86 abscinazole-F1
-
 Arabidopsis thaliana
1.14.14.86 methyl (2E)-3-[1-[(1E)-1-(4-chlorophenyl)-3-hydroxy-4,4-dimethylpent-1-en-2-yl]-1H-imidazol-5-yl]prop-2-enoate
-
 Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.86 microsome
-
 Arabidopsis thaliana
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.86 ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2 Arabidopsis thaliana
-
 ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.86 ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2 Arabidopsis thaliana Columbia
-
 ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.86 ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2 Arabidopsis thaliana
-
 ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.86 ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2 Arabidopsis thaliana Columbia
-
 ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.86 ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2 Arabidopsis thaliana
-
 ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.86 ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2 Arabidopsis thaliana Columbia
-
 ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.86 additional information Arabidopsis thaliana the enzyme catalyzes three hydroxylation reactions, which further requires dehydration at some stage, overview ?
-
 ?
1.14.14.86 additional information Arabidopsis thaliana Columbia the enzyme catalyzes three hydroxylation reactions, which further requires dehydration at some stage, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.86 Arabidopsis thaliana Q93ZB2
-
-
1.14.14.86 Arabidopsis thaliana Columbia Q93ZB2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.86 recombinant CYP701A3 partially in microsomal membranes from Escherichia coli Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.86 ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2
-
 Arabidopsis thaliana ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.86 ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2
-
 Arabidopsis thaliana Columbia ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.86 ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
-
 Arabidopsis thaliana ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.86 ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
-
 Arabidopsis thaliana Columbia ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.86 ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
-
 Arabidopsis thaliana ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.86 ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
-
 Arabidopsis thaliana Columbia ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.86 additional information the enzyme catalyzes three hydroxylation reactions, which further requires dehydration at some stage, overview Arabidopsis thaliana ?
-
 ?
1.14.14.86 additional information the enzyme catalyzes three hydroxylation reactions, which further requires dehydration at some stage, overview Arabidopsis thaliana Columbia ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.86 CYP701A3
-
 Arabidopsis thaliana
1.14.14.86 kaurene oxidase
-
 Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
1.14.14.86 metabolism kaurene oxidase is a multifunctional cytochrome P450 catalysing three sequential oxidations in gibberellin phytohormone biosynthesis. These serve to transform the C4alpha methyl of the ent-kaurene olefin intermediate into the carboxylic acid moiety of ent-kauren-19-oic acid Arabidopsis thaliana
1.14.14.86 physiological function kaurene oxidase is a multifunctional cytochrome P450 catalysing three sequential oxidations in gibberellin phytohormone biosynthesis. These serve to transform the C4alpha methyl of the ent-kaurene olefin intermediate into the carboxylic acid moiety of ent-kauren-19-oic acid Arabidopsis thaliana