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Literature summary extracted from

  • Ferreira, P.; Hernandez-Ortega, A.; Herguedas, B.; Rencoret, J.; Gutierrez, A.; Martinez, M.J.; Jimenez-Barbero, J.; Medina, M.; Martinez, A.T.
    Kinetic and chemical characterization of aldehyde oxidation by fungal aryl-alcohol oxidase (2010), Biochem. J., 425, 585-593.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.7 expressed in Escherichia coli Pleurotus eryngii

Protein Variants

EC Number Protein Variants Comment Organism
1.1.3.7 H502S the mutant shows much lower activities on 4-nitrobenzaldehyde (340fold activity decrease) than the wild type enzyme Pleurotus eryngii
1.1.3.7 H546S the mutant shows much lower activities on 4-nitrobenzaldehyde (670fold activity decrease) than the wild type enzyme Pleurotus eryngii
1.1.3.7 Y92F the mutation causes a 5fold reduction in the p-anisaldehyde kcat value Pleurotus eryngii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.7 0.7
-
 4-anisaldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
1.1.3.7 0.7
-
 3-chloro-4-anisaldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
1.1.3.7 0.8
-
 4-anisaldehyde mutant enzyme Y92F, wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
1.1.3.7 1.5
-
 3-Chlorobenzaldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
1.1.3.7 2
-
 4-nitrobenzaldehyde mutant enzyme Y92F, wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
1.1.3.7 2.2
-
 3-Fluorobenzaldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
1.1.3.7 3
-
 3,4-difluorobenzaldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
1.1.3.7 4.7
-
 4-Chlorobenzaldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
1.1.3.7 4.9
-
 4-Fluorobenzaldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
1.1.3.7 5
-
 4-nitrobenzaldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
1.1.3.7 7
-
 benzaldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
1.1.3.7 8
-
 veratraldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
1.1.3.7 13
-
 2,4-hexadienal wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.7 Pleurotus eryngii O94219
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.7 2,4-hexadien-1-ol + O2
-
 Pleurotus eryngii 2,4-hexadienal + H2O2
-
 ?
1.1.3.7 2,4-hexadienal + O2
-
 Pleurotus eryngii 2,4-hexadienoate + H2O2
-
 ?
1.1.3.7 3,4-difluorobenzaldehyde + O2
-
 Pleurotus eryngii 3,4-difluorobenzoic acid + H2O2
-
 ?
1.1.3.7 3,4-difluorobenzaldehyde + O2
-
 Pleurotus eryngii 3,4-difluorobenzoic acid + H2O2
-
 r
1.1.3.7 3-chloro-4-anisaldehyde + O2
-
 Pleurotus eryngii 3-chloro-4-anisic acid + H2O2
-
 r
1.1.3.7 3-chloro-4-anisyl alcohol + O2
-
 Pleurotus eryngii 3-chloro-4-anisaldehyde + H2O2
-
 ?
1.1.3.7 3-chlorobenzaldehyde + O2
-
 Pleurotus eryngii 3-chlorobenzoic acid + H2O2
-
 r
1.1.3.7 3-chlorobenzyl alcohol + O2
-
 Pleurotus eryngii 3-chlorobenzaldehyde + H2O2
-
 ?
1.1.3.7 3-fluorobenzaldehyde + O2
-
 Pleurotus eryngii 3-fluorobenzoic acid + H2O2
-
 r
1.1.3.7 3-fluorobenzyl alcohol + O2
-
 Pleurotus eryngii 3-fluorobenzaldehyde + H2O2
-
 ?
1.1.3.7 4-anisaldehyde + O2
-
 Pleurotus eryngii 4-anisic acid + H2O2
-
 r
1.1.3.7 4-anisyl alcohol + O2
-
 Pleurotus eryngii 4-anisaldehyde + H2O2
-
 ?
1.1.3.7 4-chlorobenzaldehyde + O2
-
 Pleurotus eryngii 4-chlorobenzoic acid + H2O2
-
 r
1.1.3.7 4-chlorobenzyl alcohol + O2
-
 Pleurotus eryngii 4-chlorobenzaldehyde + H2O2
-
 ?
1.1.3.7 4-fluorobenzaldehyde + O2
-
 Pleurotus eryngii 4-fluorobenzoic acid + H2O2
-
 r
1.1.3.7 4-fluorobenzyl alcohol + O2
-
 Pleurotus eryngii 4-fluorobenzaldehyde + H2O2
-
 ?
1.1.3.7 4-nitrobenzaldehyde + O2
-
 Pleurotus eryngii 4-nitrobenzoic acid + H2O2
-
 ?
1.1.3.7 4-nitrobenzaldehyde + O2
-
 Pleurotus eryngii 4-nitrobenzoic acid + H2O2
-
 r
1.1.3.7 benzaldehyde + O2
-
 Pleurotus eryngii benzoic acid + H2O2
-
 r
1.1.3.7 benzyl alcohol + O2
-
 Pleurotus eryngii benzaldehyde + H2O2
-
 ?
1.1.3.7 additional information AAO typically oxidizes aromatic alcohols to the corresponding aldehydes. However, the enzyme can also oxidize aromatic aldehydes to the corresponding acids Pleurotus eryngii ?
-
 ?
1.1.3.7 veratraldehyde + O2
-
 Pleurotus eryngii veratric acid + H2O2
-
 r
1.1.3.7 veratryl alcohol + O2
-
 Pleurotus eryngii veratraldehyde + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.3.7 AAO
-
 Pleurotus eryngii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.3.7 0.012
-
 4-anisaldehyde mutant enzyme Y92F, wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
1.1.3.7 0.05
-
 4-anisaldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
1.1.3.7 0.057
-
 3-chloro-4-anisaldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
1.1.3.7 0.13
-
 veratraldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
1.1.3.7 0.33
-
 2,4-hexadienal wild type enzyme, wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
1.1.3.7 0.367
-
 4-Fluorobenzaldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
1.1.3.7 0.5
-
 benzaldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
1.1.3.7 0.85
-
 3-Chlorobenzaldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
1.1.3.7 0.867
-
 3,4-difluorobenzaldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
1.1.3.7 0.883
-
 3-Fluorobenzaldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
1.1.3.7 1.05
-
 4-Chlorobenzaldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
1.1.3.7 1.21
-
 4-nitrobenzaldehyde mutant enzyme Y92F, wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
1.1.3.7 1.633
-
 4-nitrobenzaldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.7 FAD
-
 Pleurotus eryngii

General Information

EC Number General Information Comment Organism
1.1.3.7 physiological function aryl-alcohol oxidase provides H2O2 for lignin biodegradation Pleurotus eryngii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.3.7 0.013
-
 4-anisaldehyde mutant enzyme Y92F, wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
1.1.3.7 0.0167
-
 veratraldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
1.1.3.7 0.025
-
 2,4-hexadienal wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
1.1.3.7 0.073
-
 benzaldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
1.1.3.7 0.075
-
 4-Fluorobenzaldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
1.1.3.7 0.085
-
 3-chloro-4-anisaldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
1.1.3.7 0.087
-
 4-anisaldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
1.1.3.7 0.223
-
 4-Chlorobenzaldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
1.1.3.7 0.282
-
 3,4-difluorobenzaldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
1.1.3.7 0.315
-
 4-nitrobenzaldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
1.1.3.7 0.407
-
 3-Fluorobenzaldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
1.1.3.7 0.597
-
 4-nitrobenzaldehyde mutant enzyme Y92F, wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii
1.1.3.7 0.643
-
 3-Chlorobenzaldehyde wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 Pleurotus eryngii