Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Esser, J.; Rakonjac, M.; Hofmann, B.; Fischer, L.; Provost, P.; Schneider, G.; Steinhilber, D.; Samuelsson, B.; Radmark, O.
    Coactosin-like protein functions as a stabilizing chaperone for 5-lipoxygenase: Role of tryptophan 102 (2010), Biochem. J., 425, 265-274.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.11.34 coactosin-like protein binds 5-LO and promotes leukotriene formation, coactosin-like protein prevents heat-inactivation of 5-LO Homo sapiens
1.13.11.34 phosphatidylcholine
-
Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.34 expressed in Escherichia coli BL21 and MV1190 cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.34 W102A the mutation prevents interaction with coactosin-like protein, the mutant shows barely detectable dioxygenase activity Homo sapiens
1.13.11.34 W13A the mutation upregulates leukotriene production, the enzyme activity of the mutant 5-LO at low concentrations of phosphatidylcholine (0.005 mg/ml) and arachidonic acid (0.02 mM) is reduced compared to the wild type enzyme Homo sapiens
1.13.11.34 W75A the mutation upregulates leukotriene production, the enzyme activity of the mutant 5-LO at low concentrations of phosphatidylcholine (0.005 mg/ml) and arachidonic acid (0.02 mM) is reduced compared to the wild type enzyme Homo sapiens

General Stability

EC Number General Stability Organism
1.13.11.34 coactosin-like protein functions as a stabilizing chaperone for 5-lipoxygenase (preventing non-turnover inactivation) Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.13.11.34 nucleus 5-LO associates with a nuclear fraction only when differentiated cells are primed with phorbol ester and stimulated with ionophore Homo sapiens 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.34 Ca2+ activates Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.34 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.34 Mono-Mac-6 cell
-
Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.34 (6E,8Z,11Z,14Z)-(5S)-5-hydroperoxyeicosa-6,8,11,14-tetraenoate
-
Homo sapiens leukotriene A4 + H2O
-
?
1.13.11.34 arachidonate + O2
-
Homo sapiens (6E,8Z,11Z,14Z)-(5S)-hydroperoxyicosa-6,8,11,14-tetraenoate
-
?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.34 5-lipoxygenase
-
Homo sapiens
1.13.11.34 5-LO
-
Homo sapiens