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Literature summary extracted from

  • van Dalen, C.J.; Whitehouse, M.W.; Winterbourn, C.C.; Kettle, A.J.
    Thiocyanate and chloride as competing substrates for myeloperoxidase (1997), Biochem. J., 327, 487-492.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.2.2 5-thio-2-nitrobenzoic acid competitive inhibitor of myeloperoxidase Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.2.2 0.5
-
 thiocyanate in 100 mM phosphate buffer, pH 7.4, at 21°C Homo sapiens
1.11.2.2 2
-
 Br- in 100 mM phosphate buffer, pH 7.4, at 21°C Homo sapiens
1.11.2.2 175
-
 Cl- in 100 mM phosphate buffer, pH 7.4, at 21°C Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.2.2 15500
-
 SDS-PAGE under reducing conditions gives three bands with molecular masses of 57000, 39000 and 15000 Da, respectively Homo sapiens
1.11.2.2 39000
-
 SDS-PAGE under reducing conditions gives three bands with molecular masses of 57000, 39000 and 15000 Da, respectively Homo sapiens
1.11.2.2 57000
-
 SDS-PAGE under reducing conditions gives three bands with molecular masses of 57000, 39000 and 15000 Da, respectively Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.11.2.2 Cl- + H2O2 + H+ Homo sapiens physiological substrate of myeloperoxidase HClO + H2O
-
 ?
1.11.2.2 thiocyanate + H2O2 + H+ Homo sapiens thiocyanate is a major physiological substrate of myeloperoxidase and most favoured substrate compared to chloride and bromide hypothiocyanite + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.11.2.2 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.11.2.2 leukocyte
-
 Homo sapiens
-
1.11.2.2 neutrophil
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.2.2 Br- + H2O2 + H+
-
 Homo sapiens HBrO + H2O
-
 ?
1.11.2.2 Cl- + H2O2 + H+
-
 Homo sapiens HClO + H2O
-
 ?
1.11.2.2 Cl- + H2O2 + H+ physiological substrate of myeloperoxidase Homo sapiens HClO + H2O
-
 ?
1.11.2.2 I- + H2O2 + H+
-
 Homo sapiens HIO + H2O
-
 ?
1.11.2.2 thiocyanate + H2O2 + H+ thiocyanate is a major physiological substrate of myeloperoxidase and most favoured substrate compared to chloride and bromide Homo sapiens hypothiocyanite + H2O
-
 ?
1.11.2.2 thiocyanate + H2O2 + H+ thiocyanate is the most favoured substrate compared to chloride and bromide. With 0.1 mM thiocyanate about 50% of the H2O2 present is converted into hypothiocyanite, the rate of H2O2 loss catalysed by myeloperoxidase in the presence of 100 mM chloride doubles when 0.1 mM thiocyanate is added, and is maximal with 1 mM thiocyanate Homo sapiens hypothiocyanite + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.11.2.2 MPO
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.2.2 heme
-
 Homo sapiens