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Literature summary extracted from

  • Fernandes, S.; Tuohy, M.G.; Murray, P.G.
    Cloning, heterologous expression, and characterization of the xylitol and L-arabitol dehydrogenase genes, Texdh and Telad, from the thermophilic fungus Talaromyces emersonii (2010), Biochem. Genet., 48, 480-495.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.12 expression in Escherichia coli Rasamsonia emersonii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.12 0.06
-
 NAD+ pH 9, 30°C Rasamsonia emersonii
1.1.1.12 13.4
-
 L-arabinitol pH 9, 30°C Rasamsonia emersonii
1.1.1.12 23.3
-
 xylitol pH 9, 30°C Rasamsonia emersonii
1.1.1.12 483.5
-
 D-sorbitol pH 9, 30°C Rasamsonia emersonii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.12 Zn2+ structure analysis indicates that enzyme belongs to the family of Zn2+-containing, medium-chain alcohol dehydrogenases. Residues involved in Zn2+ binding are C55, H80, and E165. Thereis a second zinc-binding site C110, C113, C116, and C124 Rasamsonia emersonii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.12 41000
-
 x * 41000, calculated, x * 45000, SDS-PAGE Rasamsonia emersonii
1.1.1.12 45000
-
 x * 41000, calculated, x * 45000, SDS-PAGE Rasamsonia emersonii

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.12 Rasamsonia emersonii C5J3R8
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.12 D-sorbitol + NAD+
-
 Rasamsonia emersonii D-fructose + NADH + H+
-
 ?
1.1.1.12 L-arabinitol + NAD+
-
 Rasamsonia emersonii L-xylulose + NADH + H+
-
 ?
1.1.1.12 xylitol + NAD+
-
 Rasamsonia emersonii D-xylulose + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.12 ? x * 41000, calculated, x * 45000, SDS-PAGE Rasamsonia emersonii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.12 30 60
-
 Rasamsonia emersonii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.12 3.25
-
 xylitol pH 9, 30°C Rasamsonia emersonii
1.1.1.12 3.7
-
 D-sorbitol pH 9, 30°C Rasamsonia emersonii
1.1.1.12 4.7
-
 NAD+ pH 9, 30°C Rasamsonia emersonii
1.1.1.12 5.3
-
 L-arabinitol pH 9, 30°C Rasamsonia emersonii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.12 9
-
-
 Rasamsonia emersonii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.12 7 11
-
 Rasamsonia emersonii

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.12 additional information no activity with NADP+ Rasamsonia emersonii
1.1.1.12 NAD+ specific for NAD+ Rasamsonia emersonii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.12 0.008
-
 D-sorbitol pH 9, 30°C Rasamsonia emersonii
1.1.1.12 0.14
-
 xylitol pH 9, 30°C Rasamsonia emersonii
1.1.1.12 0.39
-
 L-arabinitol pH 9, 30°C Rasamsonia emersonii
1.1.1.12 78.3
-
 NAD+ pH 9, 30°C Rasamsonia emersonii