BRENDA - Enzyme Database

Mitochondrial and chloroplastic targeting signals of NADP+-dependent isocitrate dehydrogenase

McKinnon, D.J.; Brzezowski, P.; Wilson, K.E.; Gray, G.R.; Biochem. Cell Biol. 87, 963-974 (2009)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.1.1.42
NADP+-ICDH contains two different translational ATG start sites, fusion of mitochondrial and chloroplastic targeting signals to YFP reporter, and transient expression in Nicotiana tabacum using Agrobacterium tumefaciens transfection method. The chloroplast-tageting signal constructs are localizes in the chloroplast, while the mitochondrial constructs are localized in either in mitochondria and chloroplasts, due to an 50 amino acid additional part that is added in expression in cultivars Xanthi and Petit Havana
Nicotiana tabacum
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.42
chloroplast
-
Nicotiana tabacum
9507
-
1.1.1.42
mitochondrion
-
Nicotiana tabacum
5739
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Nicotiana tabacum
-
2-oxoglutarate + NADPH + H+ + CO2
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.42
Nicotiana tabacum
O65853
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.42
leaf
-
Nicotiana tabacum
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
711119
Nicotiana tabacum
2-oxoglutarate + NADPH + H+ + CO2
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.42
NADP+
-
Nicotiana tabacum
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.42
NADP+-ICDH contains two different translational ATG start sites, fusion of mitochondrial and chloroplastic targeting signals to YFP reporter, and transient expression in Nicotiana tabacum using Agrobacterium tumefaciens transfection method. The chloroplast-tageting signal constructs are localizes in the chloroplast, while the mitochondrial constructs are localized in either in mitochondria and chloroplasts, due to an 50 amino acid additional part that is added in expression in cultivars Xanthi and Petit Havana
Nicotiana tabacum
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.42
NADP+
-
Nicotiana tabacum
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.42
chloroplast
-
Nicotiana tabacum
9507
-
1.1.1.42
mitochondrion
-
Nicotiana tabacum
5739
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Nicotiana tabacum
-
2-oxoglutarate + NADPH + H+ + CO2
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.42
leaf
-
Nicotiana tabacum
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
711119
Nicotiana tabacum
2-oxoglutarate + NADPH + H+ + CO2
-
-
-
?