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Literature summary extracted from

  • Park, Y.H.; Patel, M.S.
    Characterization of interactions of dihydrolipoamide dehydrogenase with its binding protein in the human pyruvate dehydrogenase complex (2010), Biochem. Biophys. Res. Commun., 395, 416-419.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.1.4 expressed in Escherichia coli BL21 cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.8.1.4 D413A the mutant shows 85% activity in the forward reaction and 79% activity in the reverse reaction compared to the wild type enzyme Homo sapiens
1.8.1.4 D413N the mutant shows 119% activity in the forward reaction and 96% activity in the reverse reaction compared to the wild type enzyme Homo sapiens
1.8.1.4 H348A the mutant shows 60% activity in the forward reaction and 66% activity in the reverse reaction compared to the wild type enzyme Homo sapiens
1.8.1.4 H348L the mutant shows 65% activity in the forward reaction and 74% activity in the reverse reaction compared to the wild type enzyme Homo sapiens
1.8.1.4 R447A the mutant shows 110% activity in the forward reaction and 122% activity in the reverse reaction compared to the wild type enzyme Homo sapiens
1.8.1.4 Y438A the mutant shows 100% activity in the forward reaction and 91% activity in the reverse reaction compared to the wild type enzyme Homo sapiens
1.8.1.4 Y438F the mutant shows 100% activity in the forward reaction and 112% activity in the reverse reaction compared to the wild type enzyme Homo sapiens
1.8.1.4 Y438H the mutant shows 99% activity in the forward reaction and 92% activity in the reverse reaction compared to the wild type enzyme Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.4 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.4 Ni-NTA agarose column chromatography, gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.4 dihydrolipoamide + NAD+
-
 Homo sapiens lipoamide + NADH
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.8.1.4 E3 pyruvate dehydrogenase complex is composed of multiple copies of three catalytic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2), and dihydrolipoamide dehydrogenase (E3) Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.4 NAD+
-
 Homo sapiens