Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Tani, H.; Fujita, O.; Furuta, A.; Matsuda, Y.; Miyata, R.; Akimitsu, N.; Tanaka, J.; Tsuneda, S.; Sekiguchi, Y.; Noda, N.
    Real-time monitoring of RNA helicase activity using fluorescence resonance energy transfer in vitro (2010), Biochem. Biophys. Res. Commun., 393, 131-136.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.4.13 ATP-gamma-S 5.4 mM, about 50% of the original helicase activity is inhibited, competitive inhibitor Hepacivirus C
3.6.4.13 additional information several extracts of marine organisms exhibit different inhibitory effects on the RNA and DNA helicase activities of HCV NS3 Hepacivirus C

Organism

EC Number Organism UniProt Comment Textmining
3.6.4.13 Hepacivirus C
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.4.13 recombinant enzyme Hepacivirus C

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.4.13 additional information
-
development of continuous fluorescence assay based on fluorescence resonance energy transfer for the monitoring of RNA helicase activity in vitro. This assay will be useful for monitoring the detailed kinetics of RNA unwinding mechanisms and screening RNA helicase inhibitors at high throughput Hepacivirus C

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.4.13 ATP + H2O unwinding of dsRNA Hepacivirus C ADP + phosphate
-
?

Synonyms

EC Number Synonyms Comment Organism
3.6.4.13 HCV NS3 helicase
-
Hepacivirus C