Literature summary extracted from
Shimada, Y.; Fukuda, W.; Akada, Y.; Ishida, M.; Nakayama, J.; Imanaka, T.; Fujiwara, S.
Property of cold inducible DEAD-box RNA helicase in hyperthermophilic archaea (2009), Biochem. Biophys. Res. Commun., 389, 622-627.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.6.4.13 |
expression in Escherichia coli |
Thermococcus kodakarensis |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.6.4.13 |
Thermococcus kodakarensis |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.6.4.13 |
recombinant enzyme |
Thermococcus kodakarensis |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.6.4.13 |
ATP + H2O |
unwinding activity specific for single-strand paired RNA, does not unwind dsRNAs |
Thermococcus kodakarensis |
ADP + phosphate |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.6.4.13 |
Tk-DeaD |
- |
Thermococcus kodakarensis |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
3.6.4.13 |
50 |
- |
maximal ATPase activity and unwinding activity specific for single-strand paired RNA |
Thermococcus kodakarensis |
Temperature Range [°C]
EC Number |
Temperature Minimum [°C] |
Temperature Maximum [°C] |
Comment |
Organism |
---|
3.6.4.13 |
40 |
50 |
40°C: about 40% of maximal activity, 50°C: optimum, 60°C: less than 10% of maximal activity |
Thermococcus kodakarensis |
Temperature Stability [°C]
EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
---|
3.6.4.13 |
20 |
70 |
the enzyme starts to unfold at 20°C and fully unfolds at 70°C |
Thermococcus kodakarensis |
Expression
EC Number |
Organism |
Comment |
Expression |
---|
3.6.4.13 |
Thermococcus kodakarensis |
cold-inducible RNA helicase |
up |
3.6.4.13 |
Thermococcus kodakarensis |
the enzyme is more dominantly transcribed at 60°C than at 85°C and 93°C in both logarithmic and stationary phases. Tk-DeaD is detected only in logarithmic-phase cells cultivated at 60°C but hardly detected at 85°C and 93°C in both phases Tk-DeaD expression is, hence, post-transcriptionally regulated and appears under vigorous growth conditions at 60°C |
up |
General Information
EC Number |
General Information |
Comment |
Organism |
---|
3.6.4.13 |
physiological function |
the enzyme functions under cold stress conditions |
Thermococcus kodakarensis |