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Literature summary extracted from
- Cytochrome P450 monooxygenase from Clostridium acetobutylicum: a new alpha-fatty acid hydroxylase (2007), Biochem. Biophys. Res. Commun., 362, 114-119.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.11.2.4 | expressed in Escherichia coli BL21(DE3) cells | Clostridium acetobutylicum |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.11.2.4 | H2O2 | although a high concentration of hydrogen peroxide of 0.2 mM is necessary for high activities of the enzyme, it leads to a fast enzyme inactivation within 2-4 min | Clostridium acetobutylicum |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.11.2.4 | 48000 | - |
SDS-PAGE | Clostridium acetobutylicum |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.11.2.4 | additional information | Clostridium acetobutylicum | CYP152A2 has a clear preference for hydroxylation at alpha-position | ? | - |
? | |
| 1.11.2.4 | myristic acid + H2O2 | Clostridium acetobutylicum | - |
? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.11.2.4 | Clostridium acetobutylicum | Q97DZ0 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.11.2.4 | Ni-NTA column chromatography | Clostridium acetobutylicum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.11.2.4 | lauric acid + H2O2 | after 2 min of reaction conversion of 0.06 mM lauric acid reaches 55% | Clostridium acetobutylicum | ? | - |
? | |
| 1.11.2.4 | additional information | CYP152A2 has a clear preference for hydroxylation at alpha-position | Clostridium acetobutylicum | ? | - |
? | |
| 1.11.2.4 | additional information | even chain fatty acids with chain length between C10 and C16 are the substrates for P450CLA | Clostridium acetobutylicum | ? | - |
? | |
| 1.11.2.4 | additional information | the initial activities of P450CLA in reconstitution systems based on the use of oxygen and NADPH are either much lower than those measured with H2O2 or not measurable at all, no conversion is detected for caprylic acid and stearic acid | Clostridium acetobutylicum | ? | - |
? | |
| 1.11.2.4 | myristic acid + H2O2 | - |
Clostridium acetobutylicum | ? | - |
? | |
| 1.11.2.4 | myristic acid + H2O2 | highest activity, after 2 min of reaction conversion of 0.06 mM myristic acid reaches 40% | Clostridium acetobutylicum | ? | - |
? | |
| 1.11.2.4 | palmitic acid + H2O2 | after 2 min of reaction conversion of 0.06 mM palmitic acid reaches 60% | Clostridium acetobutylicum | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.11.2.4 | ? | x * 48000, SDS-PAGE | Clostridium acetobutylicum |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.11.2.4 | alpha-fatty acid hydroxylase | - |
Clostridium acetobutylicum |
| 1.11.2.4 | CYP152A2 | - |
Clostridium acetobutylicum |
| 1.11.2.4 | cytochrome P450 monooxygenase | - |
Clostridium acetobutylicum |
| 1.11.2.4 | P450CLA | - |
Clostridium acetobutylicum |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.11.2.4 | physiological function | CYP152A2 is a rapid hydrogen peroxide scavenging enzyme | Clostridium acetobutylicum |
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