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Literature summary extracted from

  • Gaurav, K.; Kundu, K.; Kundu, S.
    Biosynthesis of cephalosporin-C acylase enzyme: optimal media design, purification, and characterization (2010), Artif. Cells Blood Substit. Immobil. Biotechnol., 38, 277-283.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.1.93 biotechnology with an aim to increase the yield of 7-aminocephalosporanic acid production, a stepwise strategy, statistical medium is applied for optimizing the medium composition for the production of CPC acylase Micrococcus luteus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.93 additional information
-
 7-aminocephalosporanic acid estimated value: 9.43 mg/ml, pH 7.6, 34°C, Vmax: 7.65 U/ml Micrococcus luteus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.93 80000
-
-
 Micrococcus luteus

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.93 Micrococcus luteus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.93
-
 Micrococcus luteus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.93 cephalosporin C + H2O
-
 Micrococcus luteus 7-aminocephalosporanic acid + 2-amino-5-hydroxypentanoate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.93 cephalosporin-C acylase
-
 Micrococcus luteus
3.5.1.93 CPC acylase
-
 Micrococcus luteus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.93 34
-
 for the 7-aminocephalosporanic acid production Micrococcus luteus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.93 7.6
-
 for the 7-aminocephalosporanic acid production Micrococcus luteus