Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Politi, L.; Chiancone, E.; Giangiacomo, L.; Cervoni, L.; Scotto dAbusco, A.; Scorsino, S.; Scandurra, R.
    pH-, temperature- and ion-dependent oligomerization of Sulfolobus solfataricus recombinant amidase: a study with site-specific mutants (2009), Archaea, 2, 221-231.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.4 D487N site-specific mutagenesis, the D487N mutation is located in the center of the protein, far distant from the 33-48 segment. The mutant amidase, like the wild-type enzyme, responds to changes in pH and temperature with a conformational change affecting the dimer-octamer equilibrium Saccharolobus solfataricus
3.5.1.4 L34P site-specific mutagenesis, the mutant amidase, in contrast to the wild-type enzyme, is unaffected by pH and temperature remaining always in the dimeric state Saccharolobus solfataricus
3.5.1.4 T319I site-specific mutagenesis, the T319I mutation is located in a strand on the surface of the protein, which is far from, and opposite to, the N-terminal segment. The mutant amidase, like the wild-type enzyme, responds to changes in pH and temperature with a conformational change affecting the dimer-octamer equilibrium Saccharolobus solfataricus
3.5.1.4 Y41C site-specific mutagenesis, the mutant amidase, in contrast to the wild-type enzyme, is unaffected by pH and temperature remaining always in the dimeric state Saccharolobus solfataricus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.4 additional information diethylphosphoroamidate shows no inhibitory activity Saccharolobus solfataricus
3.5.1.4 phenylphosphorodiamidate competitive inhibitor, the inhibitory effect is fully reversed by SSAM substrate benzamide, demonstrating the hydrophobicity of the active site Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.4 0.125
-
 2-toluamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 0.25
-
 cyclomaltohexaose pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 0.55
-
 metacrylamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 1.225
-
 indol-3-acetamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 2.325
-
 3-phenyl-propionamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 4.975
-
 Isobutyramide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 7.25
-
 Benzamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 8.55
-
 butyramide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 12.25
-
 Propionamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 26.5
-
 acetamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.4 NaCl influences the oligomerization status of the enzyme. 60% octameric and 40% dimeric enzyme in absence of NaCl, 5% octameric and 95% dimeric enzyme at and above 150 mM NaCl, profile overview Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.4 50000
-
 2 * 50000, SDS-PAGE Saccharolobus solfataricus
3.5.1.4 110000
-
 gel filtration Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.4 Saccharolobus solfataricus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.4 2-toluamide + H2O
-
 Saccharolobus solfataricus 2-toluic acid + NH3
-
 ?
3.5.1.4 3-phenyl-propionamide + H2O
-
 Saccharolobus solfataricus 3-phenyl-propionate + NH3
-
 ?
3.5.1.4 4-methylbenzamide + H2O
-
 Saccharolobus solfataricus 4-methylbenzoic acid + NH3
-
 ?
3.5.1.4 acetamide + H2O
-
 Saccharolobus solfataricus acetate + NH3
-
 ?
3.5.1.4 benzamide + H2O
-
 Saccharolobus solfataricus benzoate + NH3
-
 ?
3.5.1.4 butyramide + H2O
-
 Saccharolobus solfataricus butyrate + NH3
-
 ?
3.5.1.4 indole-3-acetamide + H2O
-
 Saccharolobus solfataricus indole-3-acetate + NH3
-
 ?
3.5.1.4 isobutyramide + H2O
-
 Saccharolobus solfataricus isobutyrate + NH3
-
 ?
3.5.1.4 methacrylamide + H2O
-
 Saccharolobus solfataricus methacrylate + NH3
-
 ?
3.5.1.4 propionamide + H2O
-
 Saccharolobus solfataricus propionate + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.4 dimer 2 * 50000, SDS-PAGE Saccharolobus solfataricus
3.5.1.4 More at pHs above pH 7.0 only dimers are present, but with decreasing pH dimers associate into octamers with complete oligomerization occurring at pH 3.0. Oligomerization also shows reversible temperature-dependence with octamer formation increasing with temperature from 36°C to between 70°C and 80°C. Increasing salt concentrations favor dissociation of the octamers, oligomerization profiles under different conditions, mechanism, dimer structure, modelling, overview Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
3.5.1.4 SSAM
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.4 70
-
 assay at Saccharolobus solfataricus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.4 additional information
-
 wild-type and mutant enzymes, activity-temperature relationships, profiles at different pHs, overview Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.4 0.425
-
 2-toluamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 2.05
-
 indol-3-acetamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 5.75
-
 metacrylamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 6.1
-
 Benzamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 11.9
-
 cyclomaltohexaose pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 12.82
-
 3-phenyl-propionamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 19.98
-
 butyramide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 20.13
-
 acetamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 41.88
-
 Isobutyramide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 101.9
-
 Propionamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.4 5
-
 assay at Saccharolobus solfataricus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.4 additional information
-
 wild-type and mutant enzymes, activity-pH relationships, profiles at different temperatures, overview Saccharolobus solfataricus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.1.4 0.76
-
 acetamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 2.125
-
 indol-3-acetamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 2.8
-
 butyramide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 3.25
-
 2-toluamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 5.44
-
 3-phenyl-propionamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 7.41
-
 Propionamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 7.5
-
 Isobutyramide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 10.4
-
 Benzamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 11.81
-
 metacrylamide pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus
3.5.1.4 48.13
-
 cyclomaltohexaose pH 5.0, 70°C, wild-type enzyme Saccharolobus solfataricus