EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.5.5 | antimycin A | powerful inhibitor of the purified ADH complex, most likely acting at the ubiquinone acceptor site in subunit II | Komagataeibacter xylinus | |
1.1.5.5 | Myxothiazol | powerful inhibitor of the purified ADH complex, most likely acting at the ubiquinone acceptor site in subunit II | Komagataeibacter xylinus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.5.5 | 0.047 | - |
ubiquinone-1 | using ethanol as cosubstrate, pH and temperature not specified in the publication | Komagataeibacter xylinus | |
1.1.5.5 | 0.36 | - |
n-butanol | using 2,6-dichlorophenolindophenol as cosubstrate, pH 6.5, temperature not specified in the publication | Komagataeibacter xylinus | |
1.1.5.5 | 0.43 | - |
allylic alcohol | using 2,6-dichlorophenolindophenol as cosubstrate, pH 6.5, temperature not specified in the publication | Komagataeibacter xylinus | |
1.1.5.5 | 0.66 | - |
ethanol | using 2,6-dichlorophenolindophenol as cosubstrate, pH 6.5, temperature not specified in the publication | Komagataeibacter xylinus | |
1.1.5.5 | 6.9 | - |
acetaldehyde | using 2,6-dichlorophenolindophenol as cosubstrate, pH 6.5, temperature not specified in the publication | Komagataeibacter xylinus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.5.5 | membrane | - |
Komagataeibacter xylinus | 16020 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.5.5 | 41000 | - |
1 * 68000 + 1 * 41000, SDS-PAGE | Komagataeibacter xylinus |
1.1.5.5 | 68000 | - |
1 * 68000 + 1 * 41000, SDS-PAGE | Komagataeibacter xylinus |
1.1.5.5 | 119000 | - |
gel filtration | Komagataeibacter xylinus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.5.5 | Komagataeibacter xylinus | - |
- |
- |
1.1.5.5 | Komagataeibacter xylinus IFO 13693 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.5.5 | QAE-Toyopearl 550C column chromatography, DEAE-Toyopearl 650 M column chromatography, HA-Ultrogel column chromatography, and Sephacryl-S200 gel filtration | Komagataeibacter xylinus |
EC Number | Storage Stability | Organism |
---|---|---|
1.1.5.5 | 4°C, purified enzyme in 10 mM potassium phosphate buffer containing 0.1% (v/v) Triton X-100, 30 days, no appreciable loss of activity | Komagataeibacter xylinus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.5.5 | acetaldehyde + 2,6-dichlorophenolindophenol | 42% activity compared to n-butanol. The enzyme also oxidizes aldehydes, however the affinity for alcohols is at least twice as high | Komagataeibacter xylinus | ? | - |
? | |
1.1.5.5 | acetaldehyde + ferricyanide | 13% activity compared to n-butanol. The enzyme also oxidizes aldehydes, however the affinity for alcohols is at least twice as high | Komagataeibacter xylinus | ? | - |
? | |
1.1.5.5 | allylic alcohol + 2,6-dichlorophenolindophenol | 91% activity compared to n-butanol | Komagataeibacter xylinus | ? | - |
? | |
1.1.5.5 | allylic alcohol + ferricyanide | 96% activity compared to n-butanol | Komagataeibacter xylinus | ? | - |
? | |
1.1.5.5 | ethanol + 2,6-dichlorophenolindophenol | 88% activity compared to n-butanol | Komagataeibacter xylinus | acetaldehyde + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.5.5 | ethanol + 2,6-dichlorophenolindophenol | 88% activity compared to n-butanol | Komagataeibacter xylinus IFO 13693 | acetaldehyde + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.5.5 | ethanol + ferricyanide | 91% activity compared to n-butanol | Komagataeibacter xylinus | acetaldehyde + ferrocyanide | - |
? | |
1.1.5.5 | ethanol + ferricyanide | 91% activity compared to n-butanol | Komagataeibacter xylinus IFO 13693 | acetaldehyde + ferrocyanide | - |
? | |
1.1.5.5 | ethanol + phenazine methosulfate + 2,6-dichlorophenolindophenol | - |
Komagataeibacter xylinus | ? | - |
? | |
1.1.5.5 | ethanol + phenazine methosulfate + 2,6-dichlorophenolindophenol | - |
Komagataeibacter xylinus IFO 13693 | ? | - |
? | |
1.1.5.5 | ethanol + ubiquinone-1 | the ADH complex shows a high affinity for ubiquinone-1 with ethanol as cosubstrate | Komagataeibacter xylinus | acetaldehyde + ubiquinol-1 | - |
? | |
1.1.5.5 | formaldehyde + 2,6-dichlorophenolindophenol | 38% activity compared to n-butanol. The enzyme also oxidizes aldehydes, however the affinity for alcohols is at least twice as high | Komagataeibacter xylinus | ? | - |
? | |
1.1.5.5 | formaldehyde + ferricyanide | 34% activity compared to n-butanol. The enzyme also oxidizes aldehydes, however the affinity for alcohols is at least twice as high | Komagataeibacter xylinus | ? | - |
? | |
1.1.5.5 | glutaraldehyde + 2,6-dichlorophenolindophenol | 18% activity compared to n-butanol. The enzyme also oxidizes aldehydes, however the affinity for alcohols is at least twice as high | Komagataeibacter xylinus | ? | - |
? | |
1.1.5.5 | glutaraldehyde + ferricyanide | 8% activity compared to n-butanol. The enzyme also oxidizes aldehydes, however the affinity for alcohols is at least twice as high | Komagataeibacter xylinus | ? | - |
? | |
1.1.5.5 | glutaraldehyde + ferricyanide | 8% activity compared to n-butanol. The enzyme also oxidizes aldehydes, however the affinity for alcohols is at least twice as high | Komagataeibacter xylinus IFO 13693 | ? | - |
? | |
1.1.5.5 | additional information | purified ADH oxidizes primary alcohols (C2-C6) but not methanol | Komagataeibacter xylinus | ? | - |
? | |
1.1.5.5 | additional information | purified ADH oxidizes primary alcohols (C2-C6) but not methanol | Komagataeibacter xylinus IFO 13693 | ? | - |
? | |
1.1.5.5 | n-butanol + 2,6-dichlorophenolindophenol | 100% activity | Komagataeibacter xylinus | n-butanal + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.5.5 | n-propanol + ferricyanide | 98% activity compared to n-butanol | Komagataeibacter xylinus | n-propanal + ferrocyanide | - |
? | |
1.1.5.5 | n-propanol + oxidized 2,6-dichlorophenolindophenol | 96% activity compared to n-butanol | Komagataeibacter xylinus | n-propanal + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.5.5 | propionaldehyde + 2,6-dichlorophenolindophenol | 33% activity compared to n-butanol. The enzyme also oxidizes aldehydes, however the affinity for alcohols is at least twice as high | Komagataeibacter xylinus | ? | - |
? | |
1.1.5.5 | propionaldehyde + ferricyanide | 24% activity compared to n-butanol. The enzyme also oxidizes aldehydes, however the affinity for alcohols is at least twice as high | Komagataeibacter xylinus | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.5.5 | heterodimer | 1 * 68000 + 1 * 41000, SDS-PAGE | Komagataeibacter xylinus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.5.5 | ADH | - |
Komagataeibacter xylinus |
1.1.5.5 | quinohemoprotein alcohol dehydrogenase | - |
Komagataeibacter xylinus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.5.5 | 30.8 | - |
acetaldehyde | using 2,6-dichlorophenolindophenol as cosubstrate, pH 6.5, temperature not specified in the publication | Komagataeibacter xylinus | |
1.1.5.5 | 52.3 | - |
n-butanol | using 2,6-dichlorophenolindophenol as cosubstrate, pH 6.5, temperature not specified in the publication | Komagataeibacter xylinus | |
1.1.5.5 | 67.5 | - |
allylic alcohol | using 2,6-dichlorophenolindophenol as cosubstrate, pH 6.5, temperature not specified in the publication | Komagataeibacter xylinus | |
1.1.5.5 | 71 | - |
ethanol | using 2,6-dichlorophenolindophenol as cosubstrate, pH 6.5, temperature not specified in the publication | Komagataeibacter xylinus | |
1.1.5.5 | 76.3 | - |
ubiquinone-1 | using ethanol as cosubstrate, pH and temperature not specified in the publication | Komagataeibacter xylinus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.5.5 | 5.5 | - |
activity responses to pH are sharp, showing two distinct optimal pH values (pH 5.5 and 6.5) depending on the electron acceptor used (optimum pH 5.5 with ferricyanide as electron acceptor) | Komagataeibacter xylinus |
1.1.5.5 | 6.5 | - |
activity responses to pH are sharp, showing two distinct optimal pH values (pH 5.5 and 6.5) depending on the electron acceptor used (optimum pH 6.5 when phenazine methosulfate plus 2,6-dichlorophenolindophenol are used as electron acceptors) | Komagataeibacter xylinus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.5.5 | cytochrome c | the enzyme contains 4 cytochromes c per enzyme | Komagataeibacter xylinus | |
1.1.5.5 | heme | the quinohaemoprotein alcohol dehydrogenase contains heme C in both subunits, the ADH complex of contains 18 nmol of heme C per mg of protein (ratio of 3.6 mol of heme C per mol of enzyme) | Komagataeibacter xylinus | |
1.1.5.5 | pyrroloquinoline quinone | the ADH complex contains one mol of pyrroloquinoline quinone | Komagataeibacter xylinus | |
1.1.5.5 | pyrroloquinoline quinone | one pyrroloquinoline quinone is associated with one molecule of the purified ADH complex | Komagataeibacter xylinus |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
1.1.5.5 | Komagataeibacter xylinus | isoelectric focusing | - |
5.7 |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.5.5 | 108 | - |
ethanol | using 2,6-dichlorophenolindophenol as cosubstrate, pH 6.5, temperature not specified in the publication | Komagataeibacter xylinus | |
1.1.5.5 | 145 | - |
n-butanol | using 2,6-dichlorophenolindophenol as cosubstrate, pH 6.5, temperature not specified in the publication | Komagataeibacter xylinus | |
1.1.5.5 | 157 | - |
allylic alcohol | using 2,6-dichlorophenolindophenol as cosubstrate, pH 6.5, temperature not specified in the publication | Komagataeibacter xylinus | |
1.1.5.5 | 162 | - |
ubiquinone-1 | using ethanol as cosubstrate, pH and temperature not specified in the publication | Komagataeibacter xylinus | |
1.1.5.5 | 446 | - |
acetaldehyde | using 2,6-dichlorophenolindophenol as cosubstrate, pH 6.5, temperature not specified in the publication | Komagataeibacter xylinus |