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Literature summary extracted from
- Pseudomonas putida A ATCC 12633 oxidizes trimethylamine aerobically via two different pathways (2010), Arch. Microbiol., 192, 471-476.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.5.8.2 | additional information | the enzyme is not activated by AlCl3 | Pseudomonas putida |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.5.8.2 | trimethylamine | substrate inhibition | Pseudomonas putida |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.5.8.2 | 4 | - |
trimethylamine | pH 7.4, 30°C | Pseudomonas putida | |
| 1.5.8.2 | 4 | - |
trimethylamine | pH not specified in the publication, temperature not specified in the publication | Pseudomonas putida | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.5.8.1 | dimethylamine + H2O + electron-transfer flavoprotein | Pseudomonas putida | - |
methylamine + formaldehyde + reduced electron-transferring flavoprotein | - |
? | |
| 1.5.8.1 | dimethylamine + H2O + electron-transfer flavoprotein | Pseudomonas putida ATCC 12633 | - |
methylamine + formaldehyde + reduced electron-transferring flavoprotein | - |
? | |
| 1.5.8.2 | trimethylamine + H2O + electron-transfer flavoprotein | Pseudomonas putida | - |
dimethylamine + formaldehyde + reduced electron-transferring flavoprotein | - |
? | |
| 1.5.8.2 | trimethylamine + H2O + electron-transfer flavoprotein | Pseudomonas putida ATCC 12633 | - |
dimethylamine + formaldehyde + reduced electron-transferring flavoprotein | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.5.8.1 | Pseudomonas putida | - |
- |
- |
| 1.5.8.1 | Pseudomonas putida ATCC 12633 | - |
- |
- |
| 1.5.8.2 | Pseudomonas putida | - |
- |
- |
| 1.5.8.2 | Pseudomonas putida | - |
strain is able to grow on trimethylamine as a nitrogen source and trimethylamine also accumulates in the cell, slowing the bacterial growth | - |
| 1.5.8.2 | Pseudomonas putida ATCC 12633 | - |
- |
- |
| 1.5.8.2 | Pseudomonas putida ATCC 12633 | - |
strain is able to grow on trimethylamine as a nitrogen source and trimethylamine also accumulates in the cell, slowing the bacterial growth | - |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.5.8.1 | culture condition:trimethylamine-grown cell | - |
Pseudomonas putida | - |
| 1.5.8.1 | additional information | no activity in cells grown on tetradecyltrimethylammonium bromide | Pseudomonas putida | - |
| 1.5.8.2 | culture condition:tetradecyltrimethylammonium bromide-grown cell | - |
Pseudomonas putida | - |
| 1.5.8.2 | additional information | no activity in cells grown on trimethylamine | Pseudomonas putida | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.5.8.2 | 0.000031 | - |
tetradecyltrimethylammonium bromide-grown cells, pH 7.4, 30°C | Pseudomonas putida |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.5.8.1 | dimethylamine + H2O + electron-transfer flavoprotein | - |
Pseudomonas putida | methylamine + formaldehyde + reduced electron-transferring flavoprotein | - |
? | |
| 1.5.8.1 | dimethylamine + H2O + electron-transfer flavoprotein | - |
Pseudomonas putida ATCC 12633 | methylamine + formaldehyde + reduced electron-transferring flavoprotein | - |
? | |
| 1.5.8.2 | trimethylamine + H2O + electron acceptor | - |
Pseudomonas putida | dimethylamine + formaldehyde + reduced electron acceptor | - |
? | |
| 1.5.8.2 | trimethylamine + H2O + electron acceptor | - |
Pseudomonas putida ATCC 12633 | dimethylamine + formaldehyde + reduced electron acceptor | - |
? | |
| 1.5.8.2 | trimethylamine + H2O + electron-transfer flavoprotein | - |
Pseudomonas putida | dimethylamine + formaldehyde + reduced electron-transferring flavoprotein | - |
? | |
| 1.5.8.2 | trimethylamine + H2O + electron-transfer flavoprotein | - |
Pseudomonas putida ATCC 12633 | dimethylamine + formaldehyde + reduced electron-transferring flavoprotein | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.5.8.2 | TMA dehydrogenase | - |
Pseudomonas putida |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.5.8.1 | 30 | - |
assay at | Pseudomonas putida |
| 1.5.8.2 | 30 | - |
assay at | Pseudomonas putida |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.5.8.1 | 7.4 | - |
assay at | Pseudomonas putida |
| 1.5.8.2 | 7.4 | - |
assay at | Pseudomonas putida |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.5.8.1 | additional information | independent of NADPH | Pseudomonas putida | |
| 1.5.8.2 | additional information | independent of NADPH | Pseudomonas putida |
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