Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Izzo, V.; Leo, G.; Scognamiglio, R.; Troncone, L.; Birolo, L.; Di Donato, A.
    PHK from phenol hydroxylase of Pseudomonas sp. OX1. Insight into the role of an accessory protein in bacterial multicomponent monooxygenases (2011), Arch. Biochem. Biophys., 505, 48-59.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.7 expression of the phenol hydroxylase gene cluster in Escherichia coli strain JM109 in the absence or presence of the phk gene Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.7 accessory component PHK of phenol hydroxylase the accessory component of the phenol hydroxylase mediates inhibition of phenol hydroxylase activity, overview Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.7 Fe2+ PHK, the accessory component of the phenol hydroxylase, may be responsible for enhancing iron incorporation into the active site of the apo-hydroxylase Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.7 25500
-
 PHK accessory protein Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.7 phenol + NADPH + H+ + O2 Pseudomonas sp.
-
 catechol + NADP+ + H2O
-
 ?
1.14.13.7 phenol + NADPH + H+ + O2 Pseudomonas sp. OX1
-
 catechol + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.7 Pseudomonas sp.
-
-
-
1.14.13.7 Pseudomonas sp. OX1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.7 recombinant PHK, the accessory component of the phenol hydroxylase, from Escherichia coli Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.7 phenol + NADPH + H+ + O2
-
 Pseudomonas sp. catechol + NADP+ + H2O
-
 ?
1.14.13.7 phenol + NADPH + H+ + O2 monitoring the production of catechol in a continuous coupled assay with recombinant catechol 2,3-dioxygenase from Pseudomonas sp. OX1 Pseudomonas sp. catechol + NADP+ + H2O
-
 ?
1.14.13.7 phenol + NADPH + H+ + O2
-
 Pseudomonas sp. OX1 catechol + NADP+ + H2O
-
 ?
1.14.13.7 phenol + NADPH + H+ + O2 monitoring the production of catechol in a continuous coupled assay with recombinant catechol 2,3-dioxygenase from Pseudomonas sp. OX1 Pseudomonas sp. OX1 catechol + NADP+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.7 dimer 2 * 10235.27, PHK accessory protein, mass spectrometry Pseudomonas sp.
1.14.13.7 More the phenol hydroxylase complex possesses the PHL, PHN, PHO, PHM, and PHK subunits Pseudomonas sp.

Synonyms

EC Number Synonyms Comment Organism
1.14.13.7 phenol hydroxylase
-
 Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.7 25
-
 assay at Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.7 7
-
 assay at Pseudomonas sp.

General Information

EC Number General Information Comment Organism
1.14.13.7 additional information PHK, the accessory component of the phenol hydroxylase, is neither involved in the catalytic activity of the phenol hydroxylase complex nor required for the assembly of apo-hydroxylase, but may be responsible for enhancing iron incorporation into the active site of the apo-hydroxylase Pseudomonas sp.