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Literature summary extracted from

  • Rodriguez, S.B.; Stitt, B.L.; Ash, D.E.
    Cysteine 351 is an essential nucleophile in catalysis by Porphyromonas gingivalis peptidylarginine deiminase (2010), Arch. Biochem. Biophys., 504, 190-196.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.3.15 expressed in Escherichia coli BL21(DE3)pLysS cells Porphyromonas gingivalis

Protein Variants

EC Number Protein Variants Comment Organism
3.5.3.15 C351S the mutant is catalytically inactive, exhibiting less than 0.01% wild type activity Porphyromonas gingivalis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.3.15 iodoacetamide PAD exhibits pseudo first-order loss of activity in the presence of 0.1-10 mM iodoacetamide. When substrate (0.8 mM N-alpha-benzoyl-L-arginine) is present in the inactivation reaction, PAD retains 88% activity after a 4 min incubation, compared to only 32% residual activity under the same reaction conditions in the absence of substrate Porphyromonas gingivalis
3.5.3.15 iodoacetate PAD exhibits pseudo first-order loss of activity in the presence of 0.5-20 mM iodoacetate Porphyromonas gingivalis
3.5.3.15 L-canavanine suicide substrate Porphyromonas gingivalis
3.5.3.15 additional information substrate protects the enzyme from inactivation Porphyromonas gingivalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.3.15 60880
-
 calculated mass of truncated PAD with its poly-His and Xpress epitope tags Porphyromonas gingivalis
3.5.3.15 60890
-
 MALDI-TOF mass spectrometry Porphyromonas gingivalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.3.15 protein L-Arg + H2O Porphyromonas gingivalis PAD catalyzes the deimination of the guanidino group from peptidylarginine residues of various peptides to produce peptidylcitrulline and ammonia protein L-citrulline + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.15 Porphyromonas gingivalis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.15 N-alpha-benzoyl-L-arginine + H2O
-
 Porphyromonas gingivalis N-alpha-benzoyl-L-citrulline + NH3
-
 ?
3.5.3.15 protein L-Arg + H2O PAD catalyzes the deimination of the guanidino group from peptidylarginine residues of various peptides to produce peptidylcitrulline and ammonia Porphyromonas gingivalis protein L-citrulline + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.3.15 PAD
-
 Porphyromonas gingivalis
3.5.3.15 peptidylarginine deiminase
-
 Porphyromonas gingivalis