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Literature summary extracted from

  • Finnegan, S.; Yuan, H.; Wang, Y.F.; Orville, A.M.; Weber, I.T.; Gadda, G.
    Structural and kinetic studies on the Ser101Ala variant of choline oxidase: catalysis by compromise (2010), Arch. Biochem. Biophys., 501, 207-213.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.17 mutant enzyme S101A is expressed in Escherichia coli Rosetta(DE3)pLysS cells Arthrobacter globiformis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.3.17 mutant enzyme S101A, hanging drop vapor diffusion method, using 80 mM sodium cacodylate, 20% (v/v) PEG6000, 20% (v/v) glycerol, 150 mM Mg-acetate at pH 6.0 Arthrobacter globiformis

Protein Variants

EC Number Protein Variants Comment Organism
1.1.3.17 S101A mutant enzyme with increased efficiencies in the oxidative half-reactions and decreased efficiencies in the reductive half-reactions accompanied by a significant decrease in the overall rate of turnover with choline. Ser101 is important, but not essential, for catalysis Arthrobacter globiformis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.17 0.25
-
 choline wild type enzyme, in 50 mM sodium diphosphate, pH 10.0, at 25°C Arthrobacter globiformis
1.1.3.17 0.26
-
 choline mutant enzyme S101A, in 50 mM sodium diphosphate, pH 10.0, at 25°C Arthrobacter globiformis

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.17 Arthrobacter globiformis Q7X2H8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.17
-
 Arthrobacter globiformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.17 choline + O2
-
 Arthrobacter globiformis betaine aldehyde + H2O2
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.3.17 choline-oxygen 1-oxidoreductase
-
 Arthrobacter globiformis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.3.17 6.7
-
 choline mutant enzyme S101A, in 50 mM sodium diphosphate, pH 10.0, at 25°C Arthrobacter globiformis
1.1.3.17 43
-
 Betaine aldehyde mutant enzyme S101A, in 50 mM sodium diphosphate, pH 10.0, at 25°C Arthrobacter globiformis
1.1.3.17 60
-
 choline wild type enzyme, in 50 mM sodium diphosphate, pH 10.0, at 25°C Arthrobacter globiformis
1.1.3.17 133
-
 Betaine aldehyde wild type enzyme, in 50 mM sodium diphosphate, pH 10.0, at 25°C Arthrobacter globiformis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.17 FAD
-
 Arthrobacter globiformis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.3.17 25.6
-
 choline mutant enzyme S101A, in 50 mM sodium diphosphate, pH 10.0, at 25°C Arthrobacter globiformis
1.1.3.17 237
-
 choline wild type enzyme, in 50 mM sodium diphosphate, pH 10.0, at 25°C Arthrobacter globiformis