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Literature summary extracted from

  • Ralston, L.; Kwon, S.T.; Schoenbeck, M.; Ralston, J.; Schenk, D.J.; Coates, R.M.; Chappell, J.
    Cloning, heterologous expression, and functional characterization of 5-epi-aristolochene-1,3-dihydroxylase from tobacco (Nicotiana tabacum) (2001), Arch. Biochem. Biophys., 393, 222-235.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.14.149 Ancymidol dose-dependent inhibition with more than 80% by 0.075 mM ancymidol Nicotiana tabacum
1.14.14.149 ketoconazole dose-dependent inhibition with 95% inhibition at 0.1 mM ketoconazole Nicotiana tabacum

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.149 Nicotiana tabacum Q94FM7
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.149 5-epiaristolochene + 2 [reduced NADPH-hemoprotein reductase] + 2 O2
-
 Nicotiana tabacum capsidiol + 2 [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.149 5-epi-aristolochene-1,3-dihydroxylase
-
 Nicotiana tabacum
1.14.14.149 5EAH
-
 Nicotiana tabacum

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.149 heme
-
 Nicotiana tabacum

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.14.14.149 0.025
-
 pH and temperature not specified in the publication Nicotiana tabacum ketoconazole
1.14.14.149 0.025
-
 pH and temperature not specified in the publication Nicotiana tabacum Ancymidol

Expression

EC Number Organism Comment Expression
1.14.14.149 Nicotiana tabacum after an apparent lag phase of 8 h, a rapid induction of hydroxylase activity is observed 10 to 15 h after elicitor addition (cellulase or paraciticein) to the cell cultures, reaching a maximum by 18 h followed by a rather gradual decline of 10 to 20% over the next 8 h up