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Literature summary extracted from

  • Williamson, D.S.; Dent, K.C.; Weber, B.W.; Varsani, A.; Frederick, J.; Thuku, R.N.; Cameron, R.A.; van Heerden, J.H.; Cowan, D.A.; Sewell, B.T.
    Structural and biochemical characterization of a nitrilase from the thermophilic bacterium, Geobacillus pallidus RAPc8 (2010), Appl. Microbiol. Biotechnol., 88, 143-153.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.5.1 35790
-
 16 * 35790, calculated from amino acid sequence Aeribacillus pallidus
3.5.5.1 600000
-
 native enzyme, gel filtration Aeribacillus pallidus

Organism

EC Number Organism UniProt Comment Textmining
3.5.5.1 Aeribacillus pallidus Q0PIV8
-
-
3.5.5.1 Aeribacillus pallidus RAPc8 (NRRL: B-59396) Q0PIV8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.5.1 Hiprep 16/10 Q XL column chromatography and Sephacryl S-300 gel filtration Aeribacillus pallidus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.5.1 3-phenylproprionitrile + H2O 1650% substrate conversion compared to benzonitrile Aeribacillus pallidus 3-phenylpropionic acid + NH3
-
 ?
3.5.5.1 3-phenylproprionitrile + H2O 1650% substrate conversion compared to benzonitrile Aeribacillus pallidus RAPc8 (NRRL: B-59396) 3-phenylpropionic acid + NH3
-
 ?
3.5.5.1 4-chlorobenzonitrile + H2O 329% substrate conversion compared to benzonitrile Aeribacillus pallidus ?
-
 ?
3.5.5.1 4-chlorobenzonitrile + H2O 329% substrate conversion compared to benzonitrile Aeribacillus pallidus RAPc8 (NRRL: B-59396) ?
-
 ?
3.5.5.1 4-cyanopyridine + 2 H2O 1138% substrate conversion compared to benzonitrile Aeribacillus pallidus 4-pyridinecarboxylic acid + NH3
-
 ?
3.5.5.1 4-cyanopyridine + 2 H2O 1138% substrate conversion compared to benzonitrile Aeribacillus pallidus RAPc8 (NRRL: B-59396) 4-pyridinecarboxylic acid + NH3
-
 ?
3.5.5.1 benzonitrile + 2 H2O 100% substrate conversion Aeribacillus pallidus benzoic acid + NH3
-
 ?
3.5.5.1 benzonitrile + 2 H2O 100% substrate conversion Aeribacillus pallidus RAPc8 (NRRL: B-59396) benzoic acid + NH3
-
 ?
3.5.5.1 additional information the enzyme does not hydrolyze benzamide Aeribacillus pallidus ?
-
 ?
3.5.5.1 additional information the enzyme does not hydrolyze benzamide Aeribacillus pallidus RAPc8 (NRRL: B-59396) ?
-
 ?
3.5.5.1 R(+)-mandelonitrile + H2O 316% substrate conversion compared to benzonitrile Aeribacillus pallidus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.5.1 homohexadecamer 16 * 35790, calculated from amino acid sequence Aeribacillus pallidus