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Literature summary extracted from

  • Zhang, H.; Lountos, G.T.; Ching, C.B.; Jiang, R.
    Engineering of glycerol dehydrogenase for improved activity towards 1, 3-butanediol (2010), Appl. Microbiol. Biotechnol., 88, 117-124.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.6 His-tagged version expressed in Escherichia coli BL21(DE3) Escherichia coli
1.1.1.6 His-tagged version expressed in Escherichia coli BL21(DE3) Klebsiella pneumoniae
1.1.1.6 His-tagged version expressed in Escherichia coli BL21(DE3) Salmonella enterica

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.6 A15T/D16G/V17A/N19K/E23D/Q45E/S46E/T47M/V48L/E49R/F52L/K53A/V58A/V59A/Q70H/D74N/G78D/E81G/T82N/Q83K/G86T/I88V/G108N/R139S/L142M/N145R/K155Q/V256I/L260M mutant selected from a DNA shuffling library (Escherichia coli, Salmonella enterica, Klebsiella pneumoniae) Escherichia coli
1.1.1.6 D121A D121 can potentially hinder the proper binding of substrate 1,3-butanediol due to steric hindrance Escherichia coli
1.1.1.6 D16N/N19A/E23D/L28M/E30N/R31N/Q45E/S46E/V48L/E49R/F52L/K53T/D54G/V58S/G78V/I79V/T82K/A83S/I88V/G108N/R139S/L142M/N145R/K155Q/L211I/G248S/V256I/H268Y/D317E/P319L mutant selected from a DNA shuffling library (Escherichia coli, Salmonella enterica, Klebsiella pneumoniae) Escherichia coli
1.1.1.6 Q70H/D121A/G193C/E291Q/A310T mutant selected from a DNA shuffling library (Escherichia coli, Salmonella enterica, Klebsiella pneumoniae) and site-directed mutation D121A Escherichia coli
1.1.1.6 Q70H/D74N/G78D/E81G/T82N/Q83K/C84Y/G86T/I88V/G108N/E134A/E204K/L211I/E215K/I234V/V256I/L260M/E291D/S300C/A302S/E316G/V318I/A320T/I324L/T344D/P345S mutant selected from a DNA shuffling library (Escherichia coli, Salmonella enterica, Klebsiella pneumoniae) Escherichia coli
1.1.1.6 Q70H/G193C/E291Q/A310T mutant selected from a DNA shuffling library (Escherichia coli, Salmonella enterica, Klebsiella pneumoniae) Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.6 0.0165
-
 NAD+ wild type protein, pH 9.5, 30°C Escherichia coli
1.1.1.6 0.0182
-
 NAD+ mutant protein Q70H/G193C/E291Q/A310T, pH 9.5, 30°C Escherichia coli
1.1.1.6 0.4614
-
 NAD+ mutant protein Q70H/D121A/G193C/E291Q/A310T, pH 9.5, 30°C Escherichia coli
1.1.1.6 0.5946
-
 NAD+ mutant protein D121A, pH 9.5, 30°C Escherichia coli
1.1.1.6 11.9
-
 1,3-butanediol mutant protein Q70H/G193C/E291Q/A310T, pH 9.5, 30°C Escherichia coli
1.1.1.6 16.2
-
 1,3-butanediol wild type protein, pH 9.5, 30°C Escherichia coli
1.1.1.6 20.9
-
 1,3-butanediol pH 9.5, 30°C Klebsiella pneumoniae
1.1.1.6 29.2
-
 1,3-butanediol pH 9.5, 30°C Salmonella enterica
1.1.1.6 49.9
-
 1,3-butanediol mutant protein D121A, pH 9.5, 30°C Escherichia coli
1.1.1.6 77.8
-
 1,3-butanediol mutant protein Q70H/D121A/G193C/E291Q/A310T, pH 9.5, 30°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.6 Zn2+
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.6 39000
-
 x * 39000, SDS-PAGE Escherichia coli
1.1.1.6 39000
-
 x * 39000, SDS-PAGE Klebsiella pneumoniae
1.1.1.6 39000
-
 x * 39000, SDS-PAGE Salmonella enterica

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.6 Escherichia coli
-
-
-
1.1.1.6 Klebsiella pneumoniae
-
-
-
1.1.1.6 Klebsiella pneumoniae MGH78578
-
-
-
1.1.1.6 Salmonella enterica
-
-
-
1.1.1.6 Salmonella enterica ATCC 9150
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.6 immobilized metal ion affinity chromatography (Co2+) Escherichia coli
1.1.1.6 immobilized metal ion affinity chromatography (Co2+) Klebsiella pneumoniae
1.1.1.6 immobilized metal ion affinity chromatography (Co2+) Salmonella enterica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.6 3.7
-
 pH 9.5, 30°C Klebsiella pneumoniae
1.1.1.6 3.9
-
 pH 9.5, 30°C Salmonella enterica
1.1.1.6 4.8
-
 wild type protein, pH 9.5, 30°C Escherichia coli
1.1.1.6 6
-
 mutant protein D16N/N19A/E23D/L28M/E30N/R31N/Q45E/S46E/V48L/E49R/F52L/K53T/D54G/V58S/G78V/I79V/T82K/A83S/I88V/G108N/R139S/L142M/N145R/K155Q/L211I/G248S/V256I/H268Y/D317E/P319L, pH 9.5, 30°C Escherichia coli
1.1.1.6 6.8
-
 mutant protein A15T/D16G/V17A/N19K/E23D/Q45E/S46E/T47M/V48L/E49R/F52L/K53A/V58A/V59A/Q70H/D74N/G78D/E81G/T82N/Q83K/G86T/I88V/G108N/R139S/L142M/N145R/K155Q/V256I/L260M, pH 9.5, 30°C Escherichia coli
1.1.1.6 7.4
-
 mutant protein Q70H/D74N/G78D/E81G/T82N/Q83K/C84Y/G86T/I88V/G108N/E134A/E204K/L211I/E215K/I234V/V256I/L260M/E291D/S300C/A302S/E316G/V318I/A320T/I324L/T344D/P345S, pH 9.5, 30°C Escherichia coli
1.1.1.6 13.2
-
 mutant protein Q70H/G193C/E291Q/A310T, pH 9.5, 30°C Escherichia coli
1.1.1.6 90.7
-
 mutant protein D121A, pH 9.5, 30°C Escherichia coli
1.1.1.6 126.6
-
 mutant protein Q70H/D121A/G193C/E291Q/A310T, pH 9.5, 30°C Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.6 1,3-butanediol + NAD+
-
 Escherichia coli 4-hydroxy-2-butanone + NADH + H+
-
 ?
1.1.1.6 1,3-butanediol + NAD+
-
 Klebsiella pneumoniae 4-hydroxy-2-butanone + NADH + H+
-
 ?
1.1.1.6 1,3-butanediol + NAD+
-
 Salmonella enterica 4-hydroxy-2-butanone + NADH + H+
-
 ?
1.1.1.6 1,3-butanediol + NAD+
-
 Salmonella enterica ATCC 9150 4-hydroxy-2-butanone + NADH + H+
-
 ?
1.1.1.6 1,3-butanediol + NAD+
-
 Klebsiella pneumoniae MGH78578 4-hydroxy-2-butanone + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.6 ? x * 39000, SDS-PAGE Escherichia coli
1.1.1.6 ? x * 39000, SDS-PAGE Klebsiella pneumoniae
1.1.1.6 ? x * 39000, SDS-PAGE Salmonella enterica

Synonyms

EC Number Synonyms Comment Organism
1.1.1.6 glycerol dehydrogenase
-
 Escherichia coli
1.1.1.6 glycerol dehydrogenase
-
 Klebsiella pneumoniae
1.1.1.6 glycerol dehydrogenase
-
 Salmonella enterica

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.6 2.6
-
 1,3-butanediol pH 9.5, 30°C Klebsiella pneumoniae
1.1.1.6 2.7
-
 1,3-butanediol pH 9.5, 30°C Salmonella enterica
1.1.1.6 3.3
-
 1,3-butanediol wild type protein, pH 9.5, 30°C Escherichia coli
1.1.1.6 9.1
-
 1,3-butanediol mutant protein Q70H/G193C/E291Q/A310T, pH 9.5, 30°C Escherichia coli
1.1.1.6 62.4
-
 1,3-butanediol mutant protein D121A, pH 9.5, 30°C Escherichia coli
1.1.1.6 87.2
-
 1,3-butanediol mutant protein Q70H/D121A/G193C/E291Q/A310T, pH 9.5, 30°C Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.6 0.09
-
 1,3-butanediol pH 9.5, 30°C Salmonella enterica
1.1.1.6 0.12
-
 1,3-butanediol pH 9.5, 30°C Klebsiella pneumoniae
1.1.1.6 0.2
-
 1,3-butanediol wild type protein, pH 9.5, 30°C Escherichia coli
1.1.1.6 0.77
-
 1,3-butanediol mutant protein Q70H/G193C/E291Q/A310T, pH 9.5, 30°C Escherichia coli
1.1.1.6 1.12
-
 1,3-butanediol mutant protein Q70H/D121A/G193C/E291Q/A310T, pH 9.5, 30°C Escherichia coli
1.1.1.6 1.26
-
 1,3-butanediol mutant protein D121A, pH 9.5, 30°C Escherichia coli
1.1.1.6 105
-
 NAD+ mutant protein D121A, pH 9.5, 30°C Escherichia coli
1.1.1.6 188
-
 NAD+ mutant protein Q70H/D121A/G193C/E291Q/A310T, pH 9.5, 30°C Escherichia coli
1.1.1.6 200
-
 NAD+ wild type protein, pH 9.5, 30°C Escherichia coli
1.1.1.6 500
-
 NAD+ mutant protein Q70H/G193C/E291Q/A310T, pH 9.5, 30°C Escherichia coli