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Literature summary extracted from

  • Brautaset, T.; Jakobsen, O.M.; Degnes, K.F.; Netzer, R.; Naerdal, I.; Krog, A.; Dillingham, R.; Flickinger, M.C.; Ellingsen, T.E.
    Bacillus methanolicus pyruvate carboxylase and homoserine dehydrogenase I and II and their roles for L-lysine production from methanol at 50 degrees C (2010), Appl. Microbiol. Biotechnol., 87, 951-964.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.3 synthesis contrary to wild-type MGA3 cells that secrete 0.4 g/l L-lysine and 59 g/l L-glutamate under optimised fed batch methanol fermentation, the hom-1 mutant M168-20 secretes 11 g/l L-lysine and 69 g/l of L-glutamate. Overproduction of pyruvate carboxylase and its mutant enzyme P455S in M168-20 has no positive effect on the volumetric L-lysine yield and the L-lysine yield on methanol, and causes significantly reduced volumetric L-glutamate yield and L-glutamate yield on methanol Bacillus methanolicus

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.3 additional information heterologous expression in a hom-negative Escherichia coli mutant Gif 102, not able to grow on minimal medium unless added 1.5 mM of both L-threonine and L-methionine results in strains growing well on minimal agar plates without added threonine and methionine Bacillus methanolicus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.3 Bacillus methanolicus D8WXQ1 isoform hom-1
-
1.1.1.3 Bacillus methanolicus D8WXQ2 isoform hom-2
-
1.1.1.3 Bacillus methanolicus MGA3 D8WXQ1 isoform hom-1
-
1.1.1.3 Bacillus methanolicus MGA3 D8WXQ2 isoform hom-2
-

Synonyms

EC Number Synonyms Comment Organism
1.1.1.3 hom-1
-
 Bacillus methanolicus

Expression

EC Number Organism Comment Expression
1.1.1.3 Bacillus methanolicus strongly repressed by L-methionine down
1.1.1.3 Bacillus methanolicus strongly repressed by L-threonine down

General Information

EC Number General Information Comment Organism
1.1.1.3 physiological function contrary to wild-type MGA3 cells that secrete 0.4 g/l L-lysine and 59 g/l L-glutamate under optimised fed batch methanol fermentation, the hom-1 mutant M168-20 secretes 11 g/l L-lysine and 69 g/l of L-glutamate. Overproduction of pyruvate carboxylase and its mutant enzyme P455S in M168-20 has no positive effect on the volumetric L-lysine yield and the L-lysine yield on methanol, and causes significantly reduced volumetric L-glutamate yield and L-glutamate yield on methanol Bacillus methanolicus