Literature summary extracted from
Kiziak, C.; Stolz, A.
Identification of amino acid residues responsible for the enantioselectivity and amide formation capacity of the arylacetonitrilase from Pseudomonas fluorescens EBC191 (2009), Appl. Environ. Microbiol., 75, 5592-5599.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.5.5.5 |
expressed in Escherichia coli JM109 cells |
Pseudomonas fluorescens |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
3.5.5.5 |
A165E |
mutant with very low activities toward (R,S)-mandelonitrile and substrate (R,S)-2-phenylpropionitrile |
Pseudomonas fluorescens |
3.5.5.5 |
A165F |
the mutant enzyme converts racemic mandelonitrile and (R,S)-2-phenylpropionitrile to increased amounts of the R enantiomers of the corresponding acids |
Pseudomonas fluorescens |
3.5.5.5 |
A165G |
the mutant forms 4.3% amide and thus produces significantly more amide than the wild type enzyme with the substrate (R,S)-2-phenylpropionitrile |
Pseudomonas fluorescens |
3.5.5.5 |
A165H |
the mutant enzyme shows a significantly increased relative activity for mandelonitrile (compared to (R,S)-2-phenylpropionitrile) |
Pseudomonas fluorescens |
3.5.5.5 |
A165R |
mutant with very low activities toward (R,S)-mandelonitrile and substrate (R,S)-2-phenylpropionitrile |
Pseudomonas fluorescens |
3.5.5.5 |
A165W |
the mutant enzyme converts racemic mandelonitrile and (R,S)-2-phenylpropionitrile to increased amounts of the R enantiomers of the corresponding acids |
Pseudomonas fluorescens |
3.5.5.5 |
A165Y |
the mutant enzyme converts racemic mandelonitrile and (R,S)-2-phenylpropionitrile to increased amounts of the R enantiomers of the corresponding acids |
Pseudomonas fluorescens |
3.5.5.5 |
C164A |
the mutant is devoid of nitrilase activity with the substrate (R,S)-2-phenylpropionitrile |
Pseudomonas fluorescens |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.5.5.5 |
Pseudomonas fluorescens |
Q5EG61 |
strain EBC191 |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.5.5.5 |
2 (R,S)-2-phenylpropionitrile + 3 H2O |
NitP converts (R,S)-2-phenylpropionitrile to 2-phenylpropionic acid with a slight preference for the formation of the S enantiomer, the nitrilase converts only less than 1% of (R,S)-2-phenylpropionitrile to 2-phenylpropionamide |
Pseudomonas fluorescens |
2-phenylpropionic acid + 2-phenylpropionamide + NH3 |
- |
? |
|
3.5.5.5 |
2 (R,S)-mandelonitrile + 2 H2O |
NitP shows only weak enantioselectivity for the formation of (R)-mandelic acid from racemic mandelonitrile |
Pseudomonas fluorescens |
(R)-mandelic acid + (S)-mandeloamide + NH3 |
the product ratio is about 4:1 |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.5.5.5 |
NitP |
- |
Pseudomonas fluorescens |