Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Joensson, M.; Saleihan, Z.; Nes, I.F.; Holo, H.
    Construction and characterization of three lactate dehydrogenase-negative Enterococcus faecalis V583 mutants (2009), Appl. Environ. Microbiol., 75, 4901-4903.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.27 additional information construction of ldh1 and ldh-2 deletion mutants and of a catalytically inactive double knockout mutant of genes ldh-1 and ldh-2 by gene replacement achieved by double-crossover homologous recombination. The ldh-2 mutant shows hardly any differences in growth and metabolism in comparison to the wild-type. The two mutants lacking ldh-1, on the other hand, produce much less lactate than the wild-type but grew to higher cell densities and have higher final pHs, phenotypes, overview Enterococcus faecalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.27 L-lactate + NAD+ Enterococcus faecalis the wild-type V583 strain converts glucose almost exclusively to L-lactate under anaerobic conditions pyruvate + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.27 Enterococcus faecalis
-
 genes ldh-1 and ldh-2
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.27 L-lactate + NAD+
-
 Enterococcus faecalis pyruvate + NADH + H+
-
 r
1.1.1.27 L-lactate + NAD+ the wild-type V583 strain converts glucose almost exclusively to L-lactate under anaerobic conditions Enterococcus faecalis pyruvate + NADH + H+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.27 LDH-1
-
 Enterococcus faecalis
1.1.1.27 LDH-2
-
 Enterococcus faecalis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.27 NAD+
-
 Enterococcus faecalis
1.1.1.27 NADH
-
 Enterococcus faecalis

General Information

EC Number General Information Comment Organism
1.1.1.27 additional information the ldh-1 mutants detoxifies excess pyruvate by converting it to acetoin Enterococcus faecalis
1.1.1.27 physiological function although Ldh-2 contributes to lactate production, Ldh-1 plays the major role in energy metabolism in Enterococcus faecalis Enterococcus faecalis