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Literature summary extracted from

  • Steinbrenner, J.; Sandmann, G.
    Transformation of the green alga Haematococcus pluvialis with a phytoene desaturase for accelerated astaxanthin biosynthesis (2006), Appl. Environ. Microbiol., 72, 7477-7484.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.3.5.5 synthesis the norflurazon-resistant phytoene desaturase gene is used for successful nuclear transformation and genetic engineering of the carotenoid biosynthesis pathway for accelerated astaxanthin biosynthesis (astaxanthin as a food supplement for humans) Haematococcus lacustris

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.5.5 expression in Escherichia coli Haematococcus lacustris

Protein Variants

EC Number Protein Variants Comment Organism
1.3.5.5 L504R the modified phytoene desaturase is still active in conversion of phytoene to zeta-carotene and exhibits 43fold-higher resistance to the bleaching herbicide norflurazon. The norflurazon-resistant phytoene desaturase gene is used for successful nuclear transformation and genetic engineering of the carotenoid biosynthesis pathway for accelerated astaxanthin biosynthesis Haematococcus lacustris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.5.5 15-cis-phytoene + acceptor Haematococcus lacustris phytoene desaturase is an early enzyme of the carotenoid biosynthetic pathway 9,15,9'-tricis-zeta-carotene + reduced acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.5.5 Haematococcus lacustris
-
 Flotow NIES-144
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.5.5 15-cis-phytoene + acceptor
-
 Haematococcus lacustris 9,15,9'-tricis-zeta-carotene + reduced acceptor
-
 ?
1.3.5.5 15-cis-phytoene + acceptor phytoene desaturase is an early enzyme of the carotenoid biosynthetic pathway Haematococcus lacustris 9,15,9'-tricis-zeta-carotene + reduced acceptor
-
 ?