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Literature summary extracted from

  • Martinez-Hernandez, J.L.; Mata-Gomez, M.A.; Aguilar-Gonzalez, C.N.; Ilyina, A.
    A process to produce penicillin G acylase by surface-adhesion fermentation using Mucor griseocyanus to obtain 6-aminopenicillanic acid by penicillin G hydrolysis (2010), Appl. Biochem. Biotechnol., 160, 2045-2053.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.11 additional information
-
 additional information Michaelis-Menten kinetics Mucor griseocyanus
3.5.1.11 3
-
 penicillin G pH 8.0, 40°C Mucor griseocyanus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.11 extracellular
-
 Mucor griseocyanus
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.11 penicillin G + H2O Mucor griseocyanus
-
 phenylacetic acid + 6-aminopenicillanate
-
 ?
3.5.1.11 penicillin G + H2O Mucor griseocyanus H/55.1.1
-
 phenylacetic acid + 6-aminopenicillanate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.11 Mucor griseocyanus
-
-
-
3.5.1.11 Mucor griseocyanus H/55.1.1
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.11 additional information production of extracellular and mycelia-associated maPGA with Mucor griseocyanus H/55.1.1 by surface-adhesion fermentation using Opuntia imbricata, a cactus, as a natural immobilization support, 2126.50 IU/l after 48 h and 755.33 IU/l at 60 h. The immobilized PGA shows higher activity per unit volume, better operational stability, and easier synthetic applications, method optimnization and evaluation, overview Mucor griseocyanus
-
3.5.1.11 mycelium associated Mucor griseocyanus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.11 penicillin G + H2O
-
 Mucor griseocyanus phenylacetic acid + 6-aminopenicillanate
-
 ?
3.5.1.11 penicillin G + H2O hydrolysis of penicillin G to obtain 6-aminopenicillanate in a batch reactor Mucor griseocyanus phenylacetic acid + 6-aminopenicillanate detection of product 6-aminopenicillanate by reaction with p-dimethylaminobenzaldehyde ?
3.5.1.11 penicillin G + H2O
-
 Mucor griseocyanus H/55.1.1 phenylacetic acid + 6-aminopenicillanate
-
 ?
3.5.1.11 penicillin G + H2O hydrolysis of penicillin G to obtain 6-aminopenicillanate in a batch reactor Mucor griseocyanus H/55.1.1 phenylacetic acid + 6-aminopenicillanate detection of product 6-aminopenicillanate by reaction with p-dimethylaminobenzaldehyde ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.11 amidase
-
 Mucor griseocyanus
3.5.1.11 amidohydrolase
-
 Mucor griseocyanus
3.5.1.11 maPGA
-
 Mucor griseocyanus
3.5.1.11 penicillin G acylase
-
 Mucor griseocyanus
3.5.1.11 PGA
-
 Mucor griseocyanus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.11 40
-
 assay at Mucor griseocyanus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.11 8
-
 assay at Mucor griseocyanus