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Literature summary extracted from

  • Martel, C.M.; Parker, J.E.; Warrilow, A.G.; Rolley, N.J.; Kelly, S.L.; Kelly, D.E.
    Complementation of a Saccharomyces cerevisiae ERG11/CYP51 (sterol 14alpha-demethylase) doxycycline-regulated mutant and screening of the azole sensitivity of Aspergillus fumigatus isoenzymes CYP51A and CYP51B (2010), Antimicrob. Agents Chemother., 54, 4920-4923.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.154 expression of isoenzymes CYP51A and CYP51B in the Saccharomyces cerevisiae mutant strain YUG37-erg11, wherein native ERG11/CYP51 expression is controlled using a doxycycline-regulatable promoter. Aspergillus fumigatus CYP51A and CYP51B both complement Saccharomyces cerevisiae sterol 14alpha-demethylase function with comparable efficiency. In the presence of doxycycline, recombinant YUG37-pcyp51A and YUG37-pcyp51B yeasts are able to synthesize ergosterol and grow, a control strain harboring reverse-oriented cyp51A cannot. YUG37-pcyp51A and YUG37-pcyp51B constructs show identical sensitivity to itraconazole, posaconazole, clotrimazole, voriconazole, and fluconazole Aspergillus fumigatus

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.154 Aspergillus fumigatus
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isoenzymes CYP51A and CYP51B
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Synonyms

EC Number Synonyms Comment Organism
1.14.14.154 Cyp51A
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Aspergillus fumigatus
1.14.14.154 CYP51B
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Aspergillus fumigatus
1.14.14.154 sterol 14alpha-demethylase
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Aspergillus fumigatus