EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.14.154 | expression of isoenzymes CYP51A and CYP51B in the Saccharomyces cerevisiae mutant strain YUG37-erg11, wherein native ERG11/CYP51 expression is controlled using a doxycycline-regulatable promoter. Aspergillus fumigatus CYP51A and CYP51B both complement Saccharomyces cerevisiae sterol 14alpha-demethylase function with comparable efficiency. In the presence of doxycycline, recombinant YUG37-pcyp51A and YUG37-pcyp51B yeasts are able to synthesize ergosterol and grow, a control strain harboring reverse-oriented cyp51A cannot. YUG37-pcyp51A and YUG37-pcyp51B constructs show identical sensitivity to itraconazole, posaconazole, clotrimazole, voriconazole, and fluconazole | Aspergillus fumigatus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.14.154 | Aspergillus fumigatus | - |
isoenzymes CYP51A and CYP51B | - |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.14.154 | Cyp51A | - |
Aspergillus fumigatus |
1.14.14.154 | CYP51B | - |
Aspergillus fumigatus |
1.14.14.154 | sterol 14alpha-demethylase | - |
Aspergillus fumigatus |