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Literature summary extracted from

  • De Luca, F.; Giraud-Morin, C.; Rossolini, G.M.; Docquier, J.D.; Fosse, T.
    Genetic and biochemical characterization of TRU-1, the endogenous class C beta-lactamase from Aeromonas enteropelogenes (2010), Antimicrob. Agents Chemother., 54, 1547-1554.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.6 expression in Escherichia coli Aeromonas enteropelogenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.6 0.0000009
-
 cefotaxime pH 7.0, 30°C Aeromonas enteropelogenes
3.5.2.6 0.0000011
-
 cefuroxime pH 7.0, 30°C Aeromonas enteropelogenes
3.5.2.6 0.000025
-
 cefoxitin pH 7.0, 30°C Aeromonas enteropelogenes
3.5.2.6 0.00012
-
 ceftazidime pH 7.0, 30°C Aeromonas enteropelogenes
3.5.2.6 0.0002
-
 piperacillin pH 7.0, 30°C Aeromonas enteropelogenes
3.5.2.6 0.00072
-
 ampicillin pH 7.0, 30°C Aeromonas enteropelogenes
3.5.2.6 0.002
-
 penicillin G pH 7.0, 30°C Aeromonas enteropelogenes
3.5.2.6 0.006
-
 ceftriaxone pH 7.0, 30°C Aeromonas enteropelogenes
3.5.2.6 0.017
-
 cephalexin pH 7.0, 30°C Aeromonas enteropelogenes
3.5.2.6 0.045
-
 cephalothin pH 7.0, 30°C Aeromonas enteropelogenes
3.5.2.6 0.08
-
 cefazolin pH 7.0, 30°C Aeromonas enteropelogenes
3.5.2.6 0.085
-
 cefpirome pH 7.0, 30°C Aeromonas enteropelogenes
3.5.2.6 0.12
-
 cefazolin pH 7.0, 30°C Aeromonas enteropelogenes
3.5.2.6 0.36
-
 cefepime pH 7.0, 30°C Aeromonas enteropelogenes

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.6 Aeromonas enteropelogenes B2BSN6 produces only one beta-lactamase belonging to molecular class C
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.6
-
 Aeromonas enteropelogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.6 ampicillin + H2O
-
 Aeromonas enteropelogenes (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 cefazolin + H2O
-
 Aeromonas enteropelogenes (2R)-2-[(R)-carboxy[(1H-tetrazol-1-ylacetyl)amino]methyl]-5-[[(5-methyl-1,3,4-thiadiazol-2-yl)sulfanyl]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cefepime + H2O
-
 Aeromonas enteropelogenes (2R)-2-[(R)-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-5-[(1-methylpyrrolidinium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
3.5.2.6 cefotaxime + H2O
-
 Aeromonas enteropelogenes (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cefoxitin + H2O
-
 Aeromonas enteropelogenes (2R)-5-[(carbamoyloxy)methyl]-2-[(S)-carboxy(methoxy)[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cefpirome + H2O
-
 Aeromonas enteropelogenes (2S)-2-[(R)-{[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino}(carboxy)methyl]-5-[(6,7-dihydro-5H-cyclopenta[b]pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
3.5.2.6 ceftazidime + H2O
-
 Aeromonas enteropelogenes (2R)-2-[(R)-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-[[(2-carboxypropan-2-yl)oxy]imino]acetyl]amino](carboxy)methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
3.5.2.6 ceftriaxone + H2O
-
 Aeromonas enteropelogenes (2R)-2-[(R)-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-5-[[(6-hydroxy-2-methyl-5-oxo-2,5-dihydro-1,2,4-triazin-3-yl)sulfanyl]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cefuroxime + H2O
-
 Aeromonas enteropelogenes (2R)-5-[(carbamoyloxy)methyl]-2-[(R)-carboxy{[(2Z)-2-(furan-2-yl)-2-(methoxyimino)acetyl]amino}methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cephalexin + H2O
-
 Aeromonas enteropelogenes (2R)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5-methyl-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cephalothin + H2O
-
 Aeromonas enteropelogenes (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 additional information no hydrolysis of imipenem and aztreonam is detected Aeromonas enteropelogenes ?
-
 ?
3.5.2.6 penicillin G + H2O
-
 Aeromonas enteropelogenes (2R,4S)-2-[(R)-carboxy[(phenylacetyl)amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 piperacillin + H2O
-
 Aeromonas enteropelogenes (2R,4S)-2-[(R)-carboxy[[(2R)-2-[[(4-ethyl-2,3-dioxopiperazin-1-yl)carbonyl]amino]-2-phenylacetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.2.6 Cephalosporinase
-
 Aeromonas enteropelogenes
3.5.2.6 TRU-1
-
 Aeromonas enteropelogenes

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.2.6 0.0094
-
 cefepime pH 7.0, 30°C Aeromonas enteropelogenes
3.5.2.6 0.015
-
 cefotaxime pH 7.0, 30°C Aeromonas enteropelogenes
3.5.2.6 0.042
-
 cefuroxime pH 7.0, 30°C Aeromonas enteropelogenes
3.5.2.6 0.042
-
 ceftriaxone pH 7.0, 30°C Aeromonas enteropelogenes
3.5.2.6 0.11
-
 ceftazidime pH 7.0, 30°C Aeromonas enteropelogenes
3.5.2.6 0.25
-
 cefazolin pH 7.0, 30°C Aeromonas enteropelogenes
3.5.2.6 0.54
-
 piperacillin pH 7.0, 30°C Aeromonas enteropelogenes
3.5.2.6 0.55
-
 cefazolin pH 7.0, 30°C Aeromonas enteropelogenes
3.5.2.6 0.63
-
 ampicillin pH 7.0, 30°C Aeromonas enteropelogenes
3.5.2.6 0.88
-
 cefoxitin pH 7.0, 30°C Aeromonas enteropelogenes
3.5.2.6 23
-
 cefpirome pH 7.0, 30°C Aeromonas enteropelogenes
3.5.2.6 27
-
 penicillin G pH 7.0, 30°C Aeromonas enteropelogenes
3.5.2.6 160
-
 cephalexin pH 7.0, 30°C Aeromonas enteropelogenes
3.5.2.6 470
-
 cephalothin pH 7.0, 30°C Aeromonas enteropelogenes

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.2.6 3.1
-
 cefazolin pH 7.0, 30°C Aeromonas enteropelogenes
3.5.2.6 4.6
-
 cefazolin pH 7.0, 30°C Aeromonas enteropelogenes
3.5.2.6 7
-
 ceftriaxone pH 7.0, 30°C Aeromonas enteropelogenes
3.5.2.6 26
-
 cefepime pH 7.0, 30°C Aeromonas enteropelogenes
3.5.2.6 270
-
 cefpirome pH 7.0, 30°C Aeromonas enteropelogenes
3.5.2.6 880
-
 ampicillin pH 7.0, 30°C Aeromonas enteropelogenes
3.5.2.6 920
-
 ceftazidime pH 7.0, 30°C Aeromonas enteropelogenes
3.5.2.6 2700
-
 piperacillin pH 7.0, 30°C Aeromonas enteropelogenes
3.5.2.6 3800
-
 cefuroxime pH 7.0, 30°C Aeromonas enteropelogenes
3.5.2.6 9300
-
 cephalexin pH 7.0, 30°C Aeromonas enteropelogenes
3.5.2.6 10000
-
 cephalothin pH 7.0, 30°C Aeromonas enteropelogenes
3.5.2.6 14000
-
 penicillin G pH 7.0, 30°C Aeromonas enteropelogenes
3.5.2.6 17000
-
 cefotaxime pH 7.0, 30°C Aeromonas enteropelogenes
3.5.2.6 35000
-
 cefoxitin pH 7.0, 30°C Aeromonas enteropelogenes