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Literature summary extracted from

  • Krasnikov, B.F.; Nostramo, R.; Pinto, J.T.; Cooper, A.J.
    Assay and purification of omega-amidase/Nit2, a ubiquitously expressed putative tumor suppressor, that catalyzes the deamidation of the alpha-keto acid analogues of glutamine and asparagine (2009), Anal. Biochem., 391, 144-150.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.5.1.3 biotechnology development of a method to synthesize 2-oxoglutaramate which is not commercially available and development of an enzyme assay method to measure the hydrolysis of 2-oxoglutaramate and 2-oxosuccinamate in a 96-well plate format Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.3 cytosol
-
 Rattus norvegicus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.3 31000
-
 SDS-PAGE Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.3 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.3 ammonium sulfate precipitation, dialysis, DE-52 chromatography, hydroxylapatite chromatography, purified 3600fold, 15.1% recovery Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.3 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.3 126
-
 2-oxoglutaramate as substrate, pH 8.5, 37°C Rattus norvegicus
3.5.1.3 570
-
 2-oxosuccinamate as substrate, pH 8.5, 37°C Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.3 2-oxoglutaramate + H2O enzyme activity measured in a 96-well plate format Rattus norvegicus 2-oxoglutarate + NH3
-
 ?
3.5.1.3 2-oxosuccinamate + H2O enzyme activity measured in a 96-well plate format Rattus norvegicus oxaloacetate + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.3 omega-amidase/Nit2 omega-amidase has been shown to be identical to Nit2, a putative tumor suppressor protein Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.3 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.3 8.5
-
 assay at Rattus norvegicus