EC Number | Cloned (Comment) | Organism |
---|---|---|
3.5.1.114 | expressed in HEK-293T cells | Mus musculus |
3.5.1.114 | expression in HEK-293 cells | Mus musculus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.114 | 1.1 | - |
N-acetyl-S-benzyl-L-cysteine | pH 7.5, 37°C | Mus musculus | |
3.5.1.114 | 1.4 | - |
N-acetyl-L-tyrosine | pH 7.5, 37°C | Mus musculus | |
3.5.1.114 | 1.6 | - |
N-acetyl-L-phenylalanine | pH 7.5, 37°C | Mus musculus | |
3.5.1.114 | 1.8 | - |
N-acetyl-L-histidine | pH 7.5, 37°C | Mus musculus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.5.1.114 | cytoplasm | predominant cytoplasmic localization of aminoacylase III | Mus musculus | 5737 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.5.1.114 | 35000 | - |
4 * 35000, the monomers of the enzyme are arranged with a fourfold rotational symmetry, SDS-PAGE | Mus musculus |
3.5.1.114 | 35188 | - |
4 * 35188, calculated from sequence | Mus musculus |
3.5.1.114 | 140000 | - |
gel filtration | Mus musculus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.1.114 | Mus musculus | Q91XE4 | - |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.5.1.114 | additional information | the enzyme has consensus sites for N-glycosylation (Asn70 and Asn117), tyrosine sulfation (Tyr88, Tyr165, and Tyr272), protein kinase C (Thr298), and casein kinase II (Thr83, Ser130, Ser160, Thr201, and Ser266) phosphorylation, and myristylation (Gly18, Gly19, Gly22, Gly27, and Gly185) | Mus musculus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.5.1.114 | brain | less expression | Mus musculus | - |
3.5.1.114 | heart | less expression | Mus musculus | - |
3.5.1.114 | kidney | the enzyme is expressed predominantly in kidney, localized predominantly to the apical domain of S1 proximal tubules and the cytoplasm of S2 and S3 proximal tubules. The data suggest that in kidney proximal tubules, aminoacylase III plays an important role in deacetylating mercapturic acids | Mus musculus | - |
3.5.1.114 | liver | less expression | Mus musculus | - |
3.5.1.114 | lung | less expression | Mus musculus | - |
3.5.1.114 | small intestine | less expression | Mus musculus | - |
3.5.1.114 | stomach | less expression | Mus musculus | - |
3.5.1.114 | testis | less expression | Mus musculus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.114 | additional information | the enzyme uses as a substrate the N-acetylated L-aromatic amino acids tyrosine, phenylalanine, and tryptophan | Mus musculus | ? | - |
? | |
3.5.1.114 | N-acetyl-L-histidine + H2O | - |
Mus musculus | acetate + L-histidine | - |
? | |
3.5.1.114 | N-acetyl-L-phenylalanine + H2O | - |
Mus musculus | acetate + L-phenylalanine | - |
? | |
3.5.1.114 | N-acetyl-L-tyrosine + H2O | - |
Mus musculus | acetate + L-tyrosine | - |
? | |
3.5.1.114 | N-acetyl-S-benzyl-L-cysteine + H2O | - |
Mus musculus | acetate + S-benzyl-L-cysteine | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.5.1.114 | tetramer | 4 * 35000, the monomers of the enzyme are arranged with a fourfold rotational symmetry, SDS-PAGE | Mus musculus |
3.5.1.114 | tetramer | 4 * 35188, calculated from sequence | Mus musculus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.1.114 | Acy3 | - |
Mus musculus |
3.5.1.114 | aminoacylase III | - |
Mus musculus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.114 | 7.5 | - |
substrate: N-acetyl-S-benzyl-L-cysteine | Mus musculus |
3.5.1.114 | 7.6 | - |
substrate: N-acetyl-L-histidine or N-acetyl-L-tyrosine | Mus musculus |
3.5.1.114 | 7.7 | - |
substrate: N-acetyl-L-phenylalanine | Mus musculus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.5.1.114 | physiological function | aminoacylase III contributes to the nephrotoxicity to cysteine S-conjugates | Mus musculus |