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Literature summary extracted from
- Cloning and over-expression of penicillin G acylase in Escherichia coli BL21 (2010), Afr. J. Biotechnol., 9, 2697-2701.
No PubMed abstract available
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.5.1.11 | PGA gene cloning isolated from environmental and clinical samples, DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli strain DH5alpha, overexpression as C-terminally His-tagged protein in Escherichia coli strain BL21 under control of the T7 promoter using pGEM -T easy vector | Escherichia coli |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.11 | penicillin G + H2O | Escherichia coli | penicillin G acylase is a type II penicillin acylase | 6-aminopenicillanic acid + phenyl acetic acid | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.1.11 | Escherichia coli | D5M8S0 | strain isolated from environmental and clinical samples | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.11 | penicillin G + H2O | penicillin G acylase is a type II penicillin acylase | Escherichia coli | 6-aminopenicillanic acid + phenyl acetic acid | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.5.1.11 | penicillin G acylase | - |
Escherichia coli |
| 3.5.1.11 | PGA | - |
Escherichia coli |
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Release 2023.1 (January 2023)
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