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Literature summary extracted from

  • Mizutani, K.; Toyoda, M.; Sagara, K.; Takahashi, N.; Sato, A.; Kamitaka, Y.; Tsujimura, S.; Nakanishi, Y.; Sugiura, T.; Yamaguchi, S.; Kano, K.; Mikami, B.
    X-ray analysis of bilirubin oxidase from Myrothecium verrucaria at 2.3 A resolution using a twinned crystal (2010), Acta Crystallogr. Sect. F, 66, 765-770.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.3.3.5 analysis BOD has attracted considerable attention as an enzymatic catalyst for the cathode of biofuel cells that work under neutral conditions Albifimbria verrucaria
1.3.3.5 diagnostics BOD is used for diagnostic analysis of bilirubin in serum Albifimbria verrucaria

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.3.3.5 hanging drop vapour diffusion method, 0.003 ml of 9.2 mg/ml protein in 10 mM Tris-HCl, pH 8.0, are mixed with 0.003 ml of recipitant solution containing 10% 2-methyl-2,4-pentanediol, 1.44 M ammonium sulfate, 10% glycerol and 0.5 M KCl, equilibration against 0.2-1.0 ml precipitant solution at 20°C, X-ray diffraction structure determination and analysis at 2.3-2.5 A resolution, modelling Albifimbria verrucaria

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.3.3.5 Cu2+ multicopper oxidase with copper-binding sites. The type I Cu is coordinated by four amino-acid residues His398, Cys457, His462 and Met 467, and the type II Cu is coordinated by two amino-acid residues, His94 and His401, binding structure, overview Albifimbria verrucaria

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.3.5 2 bilirubin + O2 Albifimbria verrucaria
-
 2 biliverdin + 2 H2O
-
 ?
1.3.3.5 2 bilirubin + O2 Albifimbria verrucaria MT-1
-
 2 biliverdin + 2 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.3.5 Albifimbria verrucaria Q12737
-
-
1.3.3.5 Albifimbria verrucaria MT-1 Q12737
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.3.5 2 bilirubin + O2
-
 Albifimbria verrucaria 2 biliverdin + 2 H2O
-
 ?
1.3.3.5 2 bilirubin + O2
-
 Albifimbria verrucaria MT-1 2 biliverdin + 2 H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.3.5 More molecular-dynamics simulation of a BOD-mediator complex, mediator is 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), i.e. ABTS, overview Albifimbria verrucaria

Synonyms

EC Number Synonyms Comment Organism
1.3.3.5 BOD
-
 Albifimbria verrucaria