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Literature summary extracted from

  • Senda, M.; Hatta, T.; Kimbara, K.; Senda, T.
    Crystallization and preliminary crystallographic analysis of manganese(II)-dependent 2,3-dihydroxybiphenyl 1,2-dioxygenase from Bacillus sp. JF8 (2010), Acta Crystallogr. Sect. F, 66, 282-285.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.39 expressed in Escherichia coli MV1190 cells Bacillus sp. (in: Bacteria)

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.13.11.39 sitting drop vapor diffusion method, using 30% (v/v) PEG 400, 200 mM magnesium chloride, 100 mM Tris-HCl pH 8.5 or 10% (v/v) 2-propanol, 100 mM MES-NaOH pH 6.5 and 200 mM zinc acetate Bacillus sp. (in: Bacteria)

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.39 Mn2+ dependent on Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.39 36300
-
 4 * 36300, X-ray crystallography Bacillus sp. (in: Bacteria)

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.39 Bacillus sp. (in: Bacteria)
-
-
-
1.13.11.39 Bacillus sp. (in: Bacteria) JF8
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.39 DEAE Toyopearl column chromatography, phenyl Sepharose column chromatography, and Mono Q column chromatography Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.39 biphenyl-2,3-diol + O2
-
 Bacillus sp. (in: Bacteria) 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
1.13.11.39 biphenyl-2,3-diol + O2
-
 Bacillus sp. (in: Bacteria) JF8 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.39 homotetramer 4 * 36300, X-ray crystallography Bacillus sp. (in: Bacteria)

Synonyms

EC Number Synonyms Comment Organism
1.13.11.39 BphC
-
 Bacillus sp. (in: Bacteria)
1.13.11.39 manganese(II)-dependent 2,3-dihydroxybiphenyl 1,2-dioxygenase
-
 Bacillus sp. (in: Bacteria)