BRENDA - Enzyme Database

Crystallization of the Na+-translocating NADH:quinone oxidoreductase from Vibrio cholerae

Casutt, M.S.; Wendelspiess, S.; Steuber, J.; Fritz, G.; Acta Crystallogr. Sect. F 66, 1677-1679 (2010)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
7.2.1.1
hexahistidine-tagged Na+-NQR is expressed in Vibrio cholerae strain DELTAnqr lacking expression of the NQR complex
Vibrio cholerae
Crystallization (Commentary)
EC Number
Crystallization
Organism
7.2.1.1
sitting drop vapor diffusion method, using 40 mM KSCN, 21.0% PEG 2000 MME, 100 mM Tris acetate pH 8.5 and 8% (v/v) 1-propanol, cofactors such as FAD, riboflavin or NADH are not added during crystallization
Vibrio cholerae
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
7.2.1.1
membrane
-
Vibrio cholerae
16020
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
7.2.1.1
NADH + H+ + ubiquinone + n Na+/in
Vibrio cholerae
-
NAD+ + ubiquinol + n Na+/out
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
7.2.1.1
Vibrio cholerae
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
7.2.1.1
Ni-NTA agarose column chromatography and Superdex 200 gel filtration
Vibrio cholerae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7.2.1.1
NADH + H+ + ubiquinone + n Na+/in
-
710746
Vibrio cholerae
NAD+ + ubiquinol + n Na+/out
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
7.2.1.1
heterohexamer
Na+-NQR is a heterohexameric complex consisting of six different protein subunits (NqrA-NqrF)
Vibrio cholerae
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
7.2.1.1
FAD
noncovalently bound
Vibrio cholerae
7.2.1.1
FMN
two covalently bound FMNs
Vibrio cholerae
7.2.1.1
NADH
-
Vibrio cholerae
7.2.1.1
riboflavin
noncovalently bound
Vibrio cholerae
7.2.1.1
ubiquinone-8
-
Vibrio cholerae
7.2.1.1
[2Fe-2S]-center
-
Vibrio cholerae
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
7.2.1.1
hexahistidine-tagged Na+-NQR is expressed in Vibrio cholerae strain DELTAnqr lacking expression of the NQR complex
Vibrio cholerae
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
7.2.1.1
FAD
noncovalently bound
Vibrio cholerae
7.2.1.1
FMN
two covalently bound FMNs
Vibrio cholerae
7.2.1.1
NADH
-
Vibrio cholerae
7.2.1.1
riboflavin
noncovalently bound
Vibrio cholerae
7.2.1.1
ubiquinone-8
-
Vibrio cholerae
7.2.1.1
[2Fe-2S]-center
-
Vibrio cholerae
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
7.2.1.1
sitting drop vapor diffusion method, using 40 mM KSCN, 21.0% PEG 2000 MME, 100 mM Tris acetate pH 8.5 and 8% (v/v) 1-propanol, cofactors such as FAD, riboflavin or NADH are not added during crystallization
Vibrio cholerae
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
7.2.1.1
membrane
-
Vibrio cholerae
16020
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
7.2.1.1
NADH + H+ + ubiquinone + n Na+/in
Vibrio cholerae
-
NAD+ + ubiquinol + n Na+/out
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
7.2.1.1
Ni-NTA agarose column chromatography and Superdex 200 gel filtration
Vibrio cholerae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7.2.1.1
NADH + H+ + ubiquinone + n Na+/in
-
710746
Vibrio cholerae
NAD+ + ubiquinol + n Na+/out
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
7.2.1.1
heterohexamer
Na+-NQR is a heterohexameric complex consisting of six different protein subunits (NqrA-NqrF)
Vibrio cholerae