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Literature summary extracted from

  • Gabrielsen, M.; Zetterstroem, C.E.; Wang, D.; Beckham, K.S.; Elofsson, M.; Isaacs, N.W.; Roe, A.J.
    Expression, purification, crystallization and initial X-ray diffraction analysis of thiol peroxidase from Yersinia pseudotuberculosis (2010), Acta Crystallogr. Sect. F, 66, 1606-1609.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.7 expressed in Escherichia coli BL21 (deltaDE3) cells Yersinia pseudotuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.11.1.7 sitting drop vapor diffusion method, using 20% (w/v) PEG 3350, 0.2 M sodium citrate tribasic or 0.1 M sodium malonate pH 5, 12% (w/v) PEG 3350, or 12% (w/v) PEG 3350, 0.05 M Na-HEPES pH 7.0, 1% (w/v) tryptone Yersinia pseudotuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
1.11.1.7 C61S catalytically inactive Yersinia pseudotuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.7 17590
-
 calculated from amino acid sequence Yersinia pseudotuberculosis

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.7 Yersinia pseudotuberculosis Q66A71
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.7 Ni2+ HisTrap column chromatography Yersinia pseudotuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.7 guaiacol + H2O2
-
 Yersinia pseudotuberculosis tetraguaiacol + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.7 dimer x-ray crystallography Yersinia pseudotuberculosis

Synonyms

EC Number Synonyms Comment Organism
1.11.1.7 thiol peroxidase
-
 Yersinia pseudotuberculosis
1.11.1.7 Tpx Tpx is an atypical 2-Cys peroxiredoxin Yersinia pseudotuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.7 heme
-
 Yersinia pseudotuberculosis