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Literature summary extracted from

  • Akiyama, M.; Takeda, S.; Kokame, K.; Takagi, J.; Miyata, T.
    Production, crystallization and preliminary crystallographic analysis of an exosite-containing fragment of human von Willebrand factor-cleaving proteinase ADAMTS13 (2009), Acta Crystallogr. Sect. F, 65, 739-742.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.87 stable expression of His-tagged ADAMTS13 ancillary domains, ADAMTS13-DTCS, consisting of residues 287-685, in CHO Lec cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.24.87 recombinant His-tagged ADAMTS13 ancillary domains, residues 287-685, sitting-drop vapour-diffusion method, mixing of 100 nl protein solution, containing about 20 mg/ml protein, with an equal amount of reservoir solution, containing 26% w/v PEG 1500, 100 mM MES, pH 6.0, equilibration against 0.1 ml of reservoir solution, at 20°C, for 24 h, crystals are soaked in a solution containing 20% glycerol, 26% PEG 1500, 100 mM MES, pH 6.0, for cryoprotection, X-ray diffraction structure determination and analysis at 2.6-2.8 A resolution, labeling with heavy-atom derivatives Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.4.24.87 additional information replacement of signal sequence and prosequence with the mouse Nid1 signal sequence dramatically increases the secretion of ADAMTS13-DTCS into the medium Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.87 extracellular
-
Homo sapiens
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.87 45000
-
x * 45000, recombinant His-tagged ADAMTS13 ancillary domains, ADAMTS13-DTCS, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.87 von Willebrand factor + H2O Homo sapiens
-
?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.87 Homo sapiens Q76LX8
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.24.87 glycoprotein the ADAMTS13 ancillary domains, ADAMTS13-DTCS, contain four potential N-glycosylation sites Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.87 recombinant His-tagged ADAMTS13 ancillary domains, ADAMTS13-DTCS, consisting of residues 287-685, from CHO Lec cells by nickel affinity and cation exchange chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.87 plasma
-
Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.87 von Willebrand factor + H2O
-
Homo sapiens ?
-
?

Subunits

EC Number Subunits Comment Organism
3.4.24.87 ? x * 45000, recombinant His-tagged ADAMTS13 ancillary domains, ADAMTS13-DTCS, SDS-PAGE Homo sapiens
3.4.24.87 More ADAMTS13 is a multi-domain enzyme. In addition to the N-terminal metalloproteinase domain, the ancillary domains, including a disintegrin-like domain, a thrombospondin-1 type 1 repeat, a Cys-rich domain and a spacer domain, are required for von Willebrand factor recognition and cleavage Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.4.24.87 ADAMTS13
-
Homo sapiens
3.4.24.87 More ADAMTS13 is a reprolysin-type metalloproteinase belonging to the ADAMTS, i.e. a disintegrin and metalloproteinase with thrombospondin type 1 motif, family Homo sapiens
3.4.24.87 von Willebrand factor-cleaving proteinase
-
Homo sapiens

General Information

EC Number General Information Comment Organism
3.4.24.87 physiological function ADAMTS13 specifically cleaves plasma von Willebrand factor and regulates platelet adhesion and aggregation Homo sapiens