EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.24.87 | stable expression of His-tagged ADAMTS13 ancillary domains, ADAMTS13-DTCS, consisting of residues 287-685, in CHO Lec cells | Homo sapiens |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.4.24.87 | recombinant His-tagged ADAMTS13 ancillary domains, residues 287-685, sitting-drop vapour-diffusion method, mixing of 100 nl protein solution, containing about 20 mg/ml protein, with an equal amount of reservoir solution, containing 26% w/v PEG 1500, 100 mM MES, pH 6.0, equilibration against 0.1 ml of reservoir solution, at 20°C, for 24 h, crystals are soaked in a solution containing 20% glycerol, 26% PEG 1500, 100 mM MES, pH 6.0, for cryoprotection, X-ray diffraction structure determination and analysis at 2.6-2.8 A resolution, labeling with heavy-atom derivatives | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.4.24.87 | additional information | replacement of signal sequence and prosequence with the mouse Nid1 signal sequence dramatically increases the secretion of ADAMTS13-DTCS into the medium | Homo sapiens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.4.24.87 | extracellular | - |
Homo sapiens | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.24.87 | 45000 | - |
x * 45000, recombinant His-tagged ADAMTS13 ancillary domains, ADAMTS13-DTCS, SDS-PAGE | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.24.87 | von Willebrand factor + H2O | Homo sapiens | - |
? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.24.87 | Homo sapiens | Q76LX8 | - |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.4.24.87 | glycoprotein | the ADAMTS13 ancillary domains, ADAMTS13-DTCS, contain four potential N-glycosylation sites | Homo sapiens |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.24.87 | recombinant His-tagged ADAMTS13 ancillary domains, ADAMTS13-DTCS, consisting of residues 287-685, from CHO Lec cells by nickel affinity and cation exchange chromatography | Homo sapiens |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.4.24.87 | plasma | - |
Homo sapiens | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.24.87 | von Willebrand factor + H2O | - |
Homo sapiens | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.24.87 | ? | x * 45000, recombinant His-tagged ADAMTS13 ancillary domains, ADAMTS13-DTCS, SDS-PAGE | Homo sapiens |
3.4.24.87 | More | ADAMTS13 is a multi-domain enzyme. In addition to the N-terminal metalloproteinase domain, the ancillary domains, including a disintegrin-like domain, a thrombospondin-1 type 1 repeat, a Cys-rich domain and a spacer domain, are required for von Willebrand factor recognition and cleavage | Homo sapiens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.24.87 | ADAMTS13 | - |
Homo sapiens |
3.4.24.87 | More | ADAMTS13 is a reprolysin-type metalloproteinase belonging to the ADAMTS, i.e. a disintegrin and metalloproteinase with thrombospondin type 1 motif, family | Homo sapiens |
3.4.24.87 | von Willebrand factor-cleaving proteinase | - |
Homo sapiens |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.4.24.87 | physiological function | ADAMTS13 specifically cleaves plasma von Willebrand factor and regulates platelet adhesion and aggregation | Homo sapiens |