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Literature summary extracted from

  • Dong, J.B.; Liu, X.; Li, L.F.; Wu, S.; Su, X.D.
    Preliminary X-ray crystallographic analysis of a nitric oxide-inducible lactate dehydrogenase from Staphylococcus aureus (2009), Acta Crystallogr. Sect. F, 65, 1053-1055.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.1.1.27 drug development Sa-LDH-1 can become a potential drug target for antibiotics against Staphylococcus aureus Staphylococcus aureus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.27 gene ldh-1, expression of N-termially His6-tagged enzyme in Escherichia coli BL21 Staphylococcus aureus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.27 purified recombinant His-tagged Sa-LDH-1, sitting drop vapour diffusion method, 0.001 ml protein solution, containing 5 mg/ml protein in 20 mM Tris-HCl, 200 mM NaCl, pH 7.5, is mixed with mixed with an equal volume of reservoir solution, containing ,0.2 M calcium chloride dihydrate, 0.1 M Na HEPES, pH 7.5, 28%(v/v) PEG 400, and equilibrated against 0.1 ml reservoir solution, 3 days, X-ray diffraction structure determination and analysis at 2.4 A resolution Staphylococcus aureus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.27 34600
-
 x * 39000, recombinant His6-tagged LDH-1, SDS-PAGE, x * 34600, about, sequence calculation Staphylococcus aureus
1.1.1.27 39000
-
 x * 39000, recombinant His6-tagged LDH-1, SDS-PAGE, x * 34600, about, sequence calculation Staphylococcus aureus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.27 L-lactate + NAD+ Staphylococcus aureus
-
 pyruvate + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.27 Staphylococcus aureus
-
 gene ldh-1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.27 recombinant N-termially His6-tagged LDH-1 from Escherichia coli BL21 by nickel affinity chromatography and gel filtration to homogeneity Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.27 L-lactate + NAD+
-
 Staphylococcus aureus pyruvate + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.27 ? x * 39000, recombinant His6-tagged LDH-1, SDS-PAGE, x * 34600, about, sequence calculation Staphylococcus aureus

Synonyms

EC Number Synonyms Comment Organism
1.1.1.27 More lactate dehydrogenase is a member of the 2-hydroxyacid oxidoreductase family Staphylococcus aureus
1.1.1.27 nitric oxideinducible l-lactate dehydrogenase
-
 Staphylococcus aureus
1.1.1.27 Sa-LDH-1
-
 Staphylococcus aureus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.27 NAD+
-
 Staphylococcus aureus

General Information

EC Number General Information Comment Organism
1.1.1.27 physiological function the enzyme catalyzes the stereospecific conversion of lactate to pyruvate and converts NAD+ to NADH, which is an important way of regenerating NAD+, enabling the continuation of glycolysis Staphylococcus aureus