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Literature summary extracted from

  • Trofimov, A.A.; Polyakov, K.M.; Boyko, K.M.; Tikhonova, T.V.; Safonova, T.N.; Tikhonov, A.V.; Popov, A.N.; Popov, V.O.
    Structures of complexes of octahaem cytochrome c nitrite reductase from Thioalkalivibrio nitratireducens with sulfite and cyanide (2010), Acta Crystallogr. Sect. D, 66, 1043-1047.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.7.2.2 alone or in complex with nitrite and cyanide, hanging drop vapor diffusion method, using 0.2 M trisodium citrate dihydrate, 0.1 M Tris-HCl pH 8.5 and 30% (v/v) PEG 400 Thioalkalivibrio nitratireducens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.2.2 cyanide
-
 Thioalkalivibrio nitratireducens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.2.2 nitrite + acceptor Thioalkalivibrio nitratireducens
-
 NH3 + oxidized acceptor + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.2.2 Thioalkalivibrio nitratireducens Q5F2I3
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.2.2 nitrite + acceptor
-
 Thioalkalivibrio nitratireducens NH3 + oxidized acceptor + H2O
-
 ?
1.7.2.2 sulfite + reduced methyl viologen + H+
-
 Thioalkalivibrio nitratireducens H2S + oxidized methyl viologen + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.7.2.2 cytochrome c nitrite reductase
-
 Thioalkalivibrio nitratireducens
1.7.2.2 NiR
-
 Thioalkalivibrio nitratireducens
1.7.2.2 octahaem cytochrome c nitrite reductase
-
 Thioalkalivibrio nitratireducens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.2.2 7
-
 the sulfite reductase activity of NiR has a maximum value at neutral pH Thioalkalivibrio nitratireducens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.7.2.2 7 9 the sulfite reductase activity of NiR has a maximum value at neutral pH, the sulfite reductase activity of NiR decreases with increasing pH and is absent at pH 9.0, the activity measured at pH 7.8 is 20% of the activity measured at pH 7.0 Thioalkalivibrio nitratireducens

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.2.2 heme an octaheme cytochrome c nitrite reductase Thioalkalivibrio nitratireducens