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Literature summary extracted from

  • Alper, B.J.; Rowse, J.W.; Schmidt, W.K.
    Yeast Ste23p shares functional similarities with mammalian insulin-degrading enzymes (2009), Yeast, 26, 595-610.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.56 recombinant expression of His6-tagged IDE in Escherichia coli strain BL21(DE3) Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
3.4.24.56 E768A naturally occuring mutation of IDE Rattus norvegicus
3.4.24.56 Y248C naturally occuring mutation of IDE Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.56 1,10-phenanthroline
-
 Rattus norvegicus
3.4.24.56 2-mercaptoethanol
-
 Rattus norvegicus
3.4.24.56 4,7-phenanthroline
-
 Rattus norvegicus
3.4.24.56 cysteine
-
 Rattus norvegicus
3.4.24.56 DTT
-
 Rattus norvegicus
3.4.24.56 EDTA
-
 Rattus norvegicus
3.4.24.56 Insulin substrate inhibition Rattus norvegicus
3.4.24.56 additional information IDE is inhibited by metal chelators, thiol modifiers, inhibitors of cysteine protease activity and insulin, no inhibition by GTP and DMSO, poor inhibition by ATP Rattus norvegicus
3.4.24.56 NEM
-
 Rattus norvegicus
3.4.24.56 PMSF
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.56 0.219
-
 Abz-SEKKDNYIIKGV-nitroY-OH pH 9.2, 37°C, recombinant enzyme Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.56 Zn2+
-
 Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.56 amyloid beta40 + H2O Rattus norvegicus
-
 amyloid beta40 peptide fragments
-
 ?
3.4.24.56 insulin + H2O Rattus norvegicus cleavage of the B-chain insulin peptide fragments
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.56 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.56 recombinant His6-tagged IDE from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Rattus norvegicus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.56 0.22
-
 pH 9.2, 37°C, purified recombinant His-tagged enzyme, substrate Abz-SEKKDNYIIKGV-nitroY-OH Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.56 Abz-GFLRKGVQ-EDDnp + H2O
-
 Rattus norvegicus ?
-
 ?
3.4.24.56 Abz-SEKKDNYIIKGV-nitroY-OH + H2O a substrate based on the polypeptide sequence of the yeast P2 a-factor mating propheromone Rattus norvegicus ?
-
 ?
3.4.24.56 amyloid beta40 + H2O
-
 Rattus norvegicus amyloid beta40 peptide fragments
-
 ?
3.4.24.56 insulin + H2O cleavage of the B-chain Rattus norvegicus insulin peptide fragments
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.24.56 IDE
-
 Rattus norvegicus
3.4.24.56 Insulin-degrading enzyme
-
 Rattus norvegicus
3.4.24.56 More IDE belongs to the M16A enzyme family Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.56 37
-
-
 Rattus norvegicus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.24.56 25 49 temperature profile, recombinant enzyme, overview Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.56 9.2
-
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.24.56 5.8 9.2 pH profile, recombinant enzyme, overview Rattus norvegicus