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Literature summary extracted from

  • Dobrev, G.; Zhekova, B.; Delcheva, G.; Koleva, L.; Tziporkov, N.; Pishtiyski, I.
    Purification and characterization of endoxylanase Xln-1 from Aspergillus niger B03 (2009), World J. Microbiol. Biotechnol., 25, 2095-2102.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.8 Ag+ weak inhibition Aspergillus niger
3.2.1.8 Cd(CH3COO)2
-
 Aspergillus niger
3.2.1.8 Cu2+ complete inhibition at 5 mM Aspergillus niger
3.2.1.8 Fe2+
-
 Aspergillus niger
3.2.1.8 Fe3+
-
 Aspergillus niger
3.2.1.8 K+ weak inhibition Aspergillus niger
3.2.1.8 Li+ weak inhibition Aspergillus niger
3.2.1.8 Mg2+ weak inhibition Aspergillus niger
3.2.1.8 Na+ weak inhibition Aspergillus niger
3.2.1.8 Pb(CH3COO)2
-
 Aspergillus niger
3.2.1.8 Pb(NO3)2
-
 Aspergillus niger
3.2.1.8 Zn2+
-
 Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.8 additional information
-
 additional information Km for oat spelt xylan is 8.19 mg/ml, kinetics Aspergillus niger

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.8 extracellular
-
 Aspergillus niger
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.8 Co2+ activates weakly Aspergillus niger
3.2.1.8 Mn2+ activates Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 33000
-
 1 * 33000, SDS-PAGE Aspergillus niger
3.2.1.8 34000
-
 gel filtration Aspergillus niger

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.8 beta-1,4-xylan + H2O Aspergillus niger
-
 ?
-
 ?
3.2.1.8 beta-1,4-xylan + H2O Aspergillus niger B03
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Aspergillus niger
-
-
-
3.2.1.8 Aspergillus niger B03
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.8 glycoprotein the degree of glycosylation is 74.1% Aspergillus niger

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8 native enzyme 4fold by consecutive ultrafiltration and anion exchange chromatography Aspergillus niger

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.8 475.1
-
 purified native enzyme, beechwood xylan Aspergillus niger
3.2.1.8 497.2
-
 purified native enzyme, birchwood xylan Aspergillus niger
3.2.1.8 529.9
-
 purified native enzyme, oat spelt xylan Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 beta-1,4-xylan + H2O
-
 Aspergillus niger ?
-
 ?
3.2.1.8 beta-1,4-xylan + H2O from oat spelt, birchwood, or beechwood Aspergillus niger ?
-
 ?
3.2.1.8 beta-1,4-xylan + H2O
-
 Aspergillus niger B03 ?
-
 ?
3.2.1.8 beta-1,4-xylan + H2O from oat spelt, birchwood, or beechwood Aspergillus niger B03 ?
-
 ?
3.2.1.8 additional information endo mechanism of Xln-1 and substrate specificity, overview, no activity with carboxymethyl cellulose, starch, pectin, and 4-nitrophenyl-beta-D-xylopyranoside, and 4-nitrophenyl-beta-D-glucopyranoside Aspergillus niger ?
-
 ?
3.2.1.8 additional information endo mechanism of Xln-1 and substrate specificity, overview, no activity with carboxymethyl cellulose, starch, pectin, and 4-nitrophenyl-beta-D-xylopyranoside, and 4-nitrophenyl-beta-D-glucopyranoside Aspergillus niger B03 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 monomer 1 * 33000, SDS-PAGE Aspergillus niger

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 endoxylanase
-
 Aspergillus niger
3.2.1.8 Xln-1
-
 Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 60
-
-
 Aspergillus niger

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.8 30 70 activity range, profile, overview Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 40
-
 purified native enzyme, pH 7.0, 120 min, 80% activity remaining Aspergillus niger
3.2.1.8 50
-
 purified native enzyme, pH 5.0-7.0, 86% residual activity after 45 min, the crude enzyme retains only 6% of the initial activity Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 6
-
-
 Aspergillus niger

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.8 5 7 purified native enzyme, 50°C, 86% residual activity after 45 min, the crude enzyme retains only 6% of the initial activity Aspergillus niger
3.2.1.8 7
-
 purified native enzyme, 40°C, 120 min, 80% activity remaining Aspergillus niger