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Literature summary extracted from

  • Hegde, S.; Muralikrishna, G.
    Isolation and partial characterization of alkaline feruloyl esterases from Aspergillus niger CFR 1105 grown on wheat bran (2009), World J. Microbiol. Biotechnol., 25, 1963-1969.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.73 additional information activity is maximal after 4 days in solid state as well as in submerged fermentations (32.5 and 31.5 U/g dry weight of wheat bran respectively) and the enzyme titers are comparable Aspergillus niger

Application

EC Number Application Comment Organism
3.1.1.73 biotechnology ability of the enzyme to be active in alkaline pH may be advantageous in biotechnological applications and especially in the treatment of alkaline woodpulp Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.73 2
-
4-nitrophenyl ferulate purified FAE-1 Aspergillus niger
3.1.1.73 3.3
-
4-nitrophenyl ferulate purified FAE-2 Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 Aspergillus niger
-
CFR 1105
-
3.1.1.73 Aspergillus niger CFR 1105
-
CFR 1105
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.73 FAE-1 and FAE-2, to homogeneity from the day 5 culture filtrate grown in submerged fermentations, by ammonium sulphate precipitation and ion exchange chromatography, 11fold and 24.8fold, with a recovery of 10 and 8.4%, respectively Aspergillus niger

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.73 additional information
-
though the feruloyl esterase activity produced in solid state and submerged fermentations are comparable on day 5, specific activity in submerged fermentation conditions is nearly double than that of solid state ones Aspergillus niger
3.1.1.73 0.0117
-
purified FAE-2, with 4-nitrophenyl alpha-L-arabinofuranoside, 4-nitrophenyl beta-D-mannopyranoside or 4-nitrophenyl beta-D-xylopyranoside as substrates Aspergillus niger
3.1.1.73 0.015
-
in submerged fermentations at 24 h Aspergillus niger
3.1.1.73 0.0167
-
purified FAE-1, with 4-nitrophenyl butyrate, 4-nitrophenyl beta-D-galactopyranoside or 4-nitrophenyl beta-D-mannopyranoside as substrates Aspergillus niger
3.1.1.73 0.02
-
purified FAE-2, with 4-nitrophenyl beta-D-galactopyranoside as substrate Aspergillus niger
3.1.1.73 0.025
-
purified FAE-1, with 4-nitrophenyl alpha-L-arabinofuranoside or 4-nitrophenyl beta-D-xylopyranoside as substrates Aspergillus niger
3.1.1.73 0.0317
-
purified FAE-2, with 4-nitrophenyl butyrate as substrate Aspergillus niger
3.1.1.73 0.0538
-
crude extract Aspergillus niger
3.1.1.73 0.102
-
purified FAE-2, with 4-nitrophenyl acetate as substrate Aspergillus niger
3.1.1.73 0.103
-
purified FAE-1, with 4-nitrophenyl acetate as substrate Aspergillus niger
3.1.1.73 0.15
-
purified FAE-1, with 4-nitrophenyl ferulate as substrate Aspergillus niger
3.1.1.73 0.1867
-
purified FAE-2, with 4-nitrophenyl ferulate as substrate Aspergillus niger
3.1.1.73 0.195
-
on day 3 in submerged fermentations, remains constant up to 5 days Aspergillus niger
3.1.1.73 0.213
-
on day 2 in solid state fermentations, decreases thereafter Aspergillus niger
3.1.1.73 0.598
-
11fold purified FAE-1 Aspergillus niger
3.1.1.73 1.3
-
24.8fold purified FAE-2 Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.73 4-nitrophenyl acetate + H2O FAE-1 and FAE-2 Aspergillus niger acetic acid + 4-nitrophenol
-
?
3.1.1.73 4-nitrophenyl acetate + H2O FAE-1 and FAE-2 Aspergillus niger CFR 1105 acetic acid + 4-nitrophenol
-
?
3.1.1.73 4-nitrophenyl alpha-L-arabinofuranoside + H2O FAE-1 and FAE-2 Aspergillus niger alpha-L-arabinofuranose + 4-nitrophenol
-
?
3.1.1.73 4-nitrophenyl alpha-L-arabinofuranoside + H2O FAE-1 and FAE-2 Aspergillus niger CFR 1105 alpha-L-arabinofuranose + 4-nitrophenol
-
?
3.1.1.73 4-nitrophenyl beta-D-galactopyranoside + H2O FAE-1 and FAE-2 Aspergillus niger beta-D-galactopyranose + 4-nitrophenol
-
?
3.1.1.73 4-nitrophenyl beta-D-galactopyranoside + H2O FAE-1 and FAE-2 Aspergillus niger CFR 1105 beta-D-galactopyranose + 4-nitrophenol
-
?
3.1.1.73 4-nitrophenyl beta-D-mannopyranoside + H2O FAE-1 and FAE-2 Aspergillus niger beta-D-mannopyranose + 4-nitrophenol
-
?
3.1.1.73 4-nitrophenyl beta-D-mannopyranoside + H2O FAE-1 and FAE-2 Aspergillus niger CFR 1105 beta-D-mannopyranose + 4-nitrophenol
-
?
3.1.1.73 4-nitrophenyl beta-D-xylopyranoside + H2O FAE-1 and FAE-2 Aspergillus niger beta-D-xylopyranose + 4-nitrophenol
-
?
3.1.1.73 4-nitrophenyl beta-D-xylopyranoside + H2O FAE-1 and FAE-2 Aspergillus niger CFR 1105 beta-D-xylopyranose + 4-nitrophenol
-
?
3.1.1.73 4-nitrophenyl butyrate + H2O FAE-1 and FAE-2 Aspergillus niger butyric acid + 4-nitrophenol
-
?
3.1.1.73 4-nitrophenyl ferulate + H2O best substrate for FAE-1 and FAE-2 Aspergillus niger ferulic acid + 4-nitrophenol
-
?

Subunits

EC Number Subunits Comment Organism
3.1.1.73 monomer 1* 50000, SDS-PAGE, purified FAE-1. 1* 55000, SDS-PAGE, purified FAE-2 Aspergillus niger

Synonyms

EC Number Synonyms Comment Organism
3.1.1.73 Fae-1
-
Aspergillus niger
3.1.1.73 FAE-2
-
Aspergillus niger
3.1.1.73 feruloyl esterase
-
Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.73 40
-
purified FAE-1 Aspergillus niger
3.1.1.73 40 50 purified FAE-2 Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.73 30 40 60% of the activities are retained when FAE-1 and FAE-2 are preincubated at 50°C for 30 min Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.73 6
-
purified FAE-2 Aspergillus niger
3.1.1.73 9
-
purified FAE-1 Aspergillus niger

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.73 5 10 FAE-1 shows about 20% activity at pH 4.0, ca. 70% activity at pH 6.0, ca. 85% activity at pH 8.0 and ca. 50% activity at pH 10.0 and. FAE-2 shows about 10% activity at pH 4.0, ca. 50% activity at pH 6.0 and pH 10, ca. 75% activity at pH 8.0 and retains about 60% at pH 9.0 Aspergillus niger

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.73 10
-
both FAE-1 and FAE-2 are stable at alkaline pH, retaining ca. 50% of their original activity at pH 10 after 2 h of incubation Aspergillus niger