EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.73 | additional information | activity is maximal after 4 days in solid state as well as in submerged fermentations (32.5 and 31.5 U/g dry weight of wheat bran respectively) and the enzyme titers are comparable | Aspergillus niger |
EC Number | Application | Comment | Organism |
---|---|---|---|
3.1.1.73 | biotechnology | ability of the enzyme to be active in alkaline pH may be advantageous in biotechnological applications and especially in the treatment of alkaline woodpulp | Aspergillus niger |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.73 | 2 | - |
4-nitrophenyl ferulate | purified FAE-1 | Aspergillus niger | |
3.1.1.73 | 3.3 | - |
4-nitrophenyl ferulate | purified FAE-2 | Aspergillus niger |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.73 | Aspergillus niger | - |
CFR 1105 | - |
3.1.1.73 | Aspergillus niger CFR 1105 | - |
CFR 1105 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.73 | FAE-1 and FAE-2, to homogeneity from the day 5 culture filtrate grown in submerged fermentations, by ammonium sulphate precipitation and ion exchange chromatography, 11fold and 24.8fold, with a recovery of 10 and 8.4%, respectively | Aspergillus niger |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.1.73 | additional information | - |
though the feruloyl esterase activity produced in solid state and submerged fermentations are comparable on day 5, specific activity in submerged fermentation conditions is nearly double than that of solid state ones | Aspergillus niger |
3.1.1.73 | 0.0117 | - |
purified FAE-2, with 4-nitrophenyl alpha-L-arabinofuranoside, 4-nitrophenyl beta-D-mannopyranoside or 4-nitrophenyl beta-D-xylopyranoside as substrates | Aspergillus niger |
3.1.1.73 | 0.015 | - |
in submerged fermentations at 24 h | Aspergillus niger |
3.1.1.73 | 0.0167 | - |
purified FAE-1, with 4-nitrophenyl butyrate, 4-nitrophenyl beta-D-galactopyranoside or 4-nitrophenyl beta-D-mannopyranoside as substrates | Aspergillus niger |
3.1.1.73 | 0.02 | - |
purified FAE-2, with 4-nitrophenyl beta-D-galactopyranoside as substrate | Aspergillus niger |
3.1.1.73 | 0.025 | - |
purified FAE-1, with 4-nitrophenyl alpha-L-arabinofuranoside or 4-nitrophenyl beta-D-xylopyranoside as substrates | Aspergillus niger |
3.1.1.73 | 0.0317 | - |
purified FAE-2, with 4-nitrophenyl butyrate as substrate | Aspergillus niger |
3.1.1.73 | 0.0538 | - |
crude extract | Aspergillus niger |
3.1.1.73 | 0.102 | - |
purified FAE-2, with 4-nitrophenyl acetate as substrate | Aspergillus niger |
3.1.1.73 | 0.103 | - |
purified FAE-1, with 4-nitrophenyl acetate as substrate | Aspergillus niger |
3.1.1.73 | 0.15 | - |
purified FAE-1, with 4-nitrophenyl ferulate as substrate | Aspergillus niger |
3.1.1.73 | 0.1867 | - |
purified FAE-2, with 4-nitrophenyl ferulate as substrate | Aspergillus niger |
3.1.1.73 | 0.195 | - |
on day 3 in submerged fermentations, remains constant up to 5 days | Aspergillus niger |
3.1.1.73 | 0.213 | - |
on day 2 in solid state fermentations, decreases thereafter | Aspergillus niger |
3.1.1.73 | 0.598 | - |
11fold purified FAE-1 | Aspergillus niger |
3.1.1.73 | 1.3 | - |
24.8fold purified FAE-2 | Aspergillus niger |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.73 | 4-nitrophenyl acetate + H2O | FAE-1 and FAE-2 | Aspergillus niger | acetic acid + 4-nitrophenol | - |
? | |
3.1.1.73 | 4-nitrophenyl acetate + H2O | FAE-1 and FAE-2 | Aspergillus niger CFR 1105 | acetic acid + 4-nitrophenol | - |
? | |
3.1.1.73 | 4-nitrophenyl alpha-L-arabinofuranoside + H2O | FAE-1 and FAE-2 | Aspergillus niger | alpha-L-arabinofuranose + 4-nitrophenol | - |
? | |
3.1.1.73 | 4-nitrophenyl alpha-L-arabinofuranoside + H2O | FAE-1 and FAE-2 | Aspergillus niger CFR 1105 | alpha-L-arabinofuranose + 4-nitrophenol | - |
? | |
3.1.1.73 | 4-nitrophenyl beta-D-galactopyranoside + H2O | FAE-1 and FAE-2 | Aspergillus niger | beta-D-galactopyranose + 4-nitrophenol | - |
? | |
3.1.1.73 | 4-nitrophenyl beta-D-galactopyranoside + H2O | FAE-1 and FAE-2 | Aspergillus niger CFR 1105 | beta-D-galactopyranose + 4-nitrophenol | - |
? | |
3.1.1.73 | 4-nitrophenyl beta-D-mannopyranoside + H2O | FAE-1 and FAE-2 | Aspergillus niger | beta-D-mannopyranose + 4-nitrophenol | - |
? | |
3.1.1.73 | 4-nitrophenyl beta-D-mannopyranoside + H2O | FAE-1 and FAE-2 | Aspergillus niger CFR 1105 | beta-D-mannopyranose + 4-nitrophenol | - |
? | |
3.1.1.73 | 4-nitrophenyl beta-D-xylopyranoside + H2O | FAE-1 and FAE-2 | Aspergillus niger | beta-D-xylopyranose + 4-nitrophenol | - |
? | |
3.1.1.73 | 4-nitrophenyl beta-D-xylopyranoside + H2O | FAE-1 and FAE-2 | Aspergillus niger CFR 1105 | beta-D-xylopyranose + 4-nitrophenol | - |
? | |
3.1.1.73 | 4-nitrophenyl butyrate + H2O | FAE-1 and FAE-2 | Aspergillus niger | butyric acid + 4-nitrophenol | - |
? | |
3.1.1.73 | 4-nitrophenyl ferulate + H2O | best substrate for FAE-1 and FAE-2 | Aspergillus niger | ferulic acid + 4-nitrophenol | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.1.73 | monomer | 1* 50000, SDS-PAGE, purified FAE-1. 1* 55000, SDS-PAGE, purified FAE-2 | Aspergillus niger |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.73 | Fae-1 | - |
Aspergillus niger |
3.1.1.73 | FAE-2 | - |
Aspergillus niger |
3.1.1.73 | feruloyl esterase | - |
Aspergillus niger |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.73 | 40 | - |
purified FAE-1 | Aspergillus niger |
3.1.1.73 | 40 | 50 | purified FAE-2 | Aspergillus niger |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.73 | 30 | 40 | 60% of the activities are retained when FAE-1 and FAE-2 are preincubated at 50°C for 30 min | Aspergillus niger |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.73 | 6 | - |
purified FAE-2 | Aspergillus niger |
3.1.1.73 | 9 | - |
purified FAE-1 | Aspergillus niger |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.73 | 5 | 10 | FAE-1 shows about 20% activity at pH 4.0, ca. 70% activity at pH 6.0, ca. 85% activity at pH 8.0 and ca. 50% activity at pH 10.0 and. FAE-2 shows about 10% activity at pH 4.0, ca. 50% activity at pH 6.0 and pH 10, ca. 75% activity at pH 8.0 and retains about 60% at pH 9.0 | Aspergillus niger |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.73 | 10 | - |
both FAE-1 and FAE-2 are stable at alkaline pH, retaining ca. 50% of their original activity at pH 10 after 2 h of incubation | Aspergillus niger |