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Literature summary extracted from

  • Lodha, P.H.; Jaworski, A.F.; Aitken, S.M.
    Characterization of site-directed mutants of residues R58, R59, D116, W340 and R372 in the active site of E. coli cystathionine beta-lyase (2010), Protein Sci., 19, 383-391.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.13 mutant enzymes are expressed in the Escherichia coli KS1000 metC:cat strain Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
4.4.1.13 D116A mutant with reduced catalytic efficiency Escherichia coli
4.4.1.13 D116N mutant with reduced catalytic efficiency Escherichia coli
4.4.1.13 R372A mutant with reduced catalytic efficiency Escherichia coli
4.4.1.13 R372K mutant with reduced catalytic efficiency Escherichia coli
4.4.1.13 R372L mutant with reduced catalytic efficiency Escherichia coli
4.4.1.13 R58A mutant with reduced catalytic efficiency Escherichia coli
4.4.1.13 R58K mutant with reduced catalytic efficiency Escherichia coli
4.4.1.13 R59A mutant with reduced catalytic efficiency Escherichia coli
4.4.1.13 R59K mutant with reduced catalytic efficiency Escherichia coli
4.4.1.13 W340F mutant with reduced catalytic efficiency Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.4.1.13 aminoethoxyvinylglycine
-
 Escherichia coli
4.4.1.13 N-hydrazinocarbonylmethyl-2-nitrobenzamide
-
 Escherichia coli
4.4.1.13 N-hydrazinocarbonylmethyl-2-trifluoromethylbenzamide
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.13 0.18
-
 L-cystathionine wild type enzyme, at 25°C Escherichia coli
4.4.1.13 0.55
-
 L-cystathionine mutant enzyme D116N, at 25°C Escherichia coli
4.4.1.13 0.67
-
 L-cystathionine mutant enzyme D116A, at 25°C Escherichia coli
4.4.1.13 1.02
-
 L-cystathionine mutant enzyme R59A, at 25°C Escherichia coli
4.4.1.13 1.35
-
 L-cystathionine mutant enzyme W340F, at 25°C Escherichia coli
4.4.1.13 1.51
-
 L-cystathionine mutant enzyme R59K, at 25°C Escherichia coli
4.4.1.13 5.1
-
 L-cystathionine mutant enzyme R58A, at 25°C Escherichia coli
4.4.1.13 15
-
 L-cystathionine mutant enzyme R372K, at 25°C Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.13 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.13
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.13 L-cystathionine + H2O
-
 Escherichia coli L-homocysteine + NH3 + pyruvate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.4.1.13 CBL
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.4.1.13 8.1
-
 L-cystathionine mutant enzyme R372K, at 25°C Escherichia coli
4.4.1.13 9.7
-
 L-cystathionine mutant enzyme R58A, at 25°C Escherichia coli
4.4.1.13 34.1
-
 L-cystathionine wild type enzyme, at 25°C Escherichia coli
4.4.1.13 36.7
-
 L-cystathionine mutant enzyme R59K, at 25°C Escherichia coli
4.4.1.13 37.9
-
 L-cystathionine mutant enzyme D116N, at 25°C Escherichia coli
4.4.1.13 41.9
-
 L-cystathionine mutant enzyme D116A, at 25°C Escherichia coli
4.4.1.13 45.8
-
 L-cystathionine mutant enzyme R59A, at 25°C Escherichia coli
4.4.1.13 79
-
 L-cystathionine mutant enzyme W340F, at 25°C Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
4.4.1.13 pyridoxal 5'-phosphate
-
 Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.4.1.13 0.0019
-
 aminoethoxyvinylglycine wild type enzyme, at 25°C Escherichia coli
4.4.1.13 0.0027
-
 aminoethoxyvinylglycine mutant enzyme D116N, at 25°C Escherichia coli
4.4.1.13 0.0027
-
 aminoethoxyvinylglycine mutant enzyme R59A, at 25°C Escherichia coli
4.4.1.13 0.0039
-
 aminoethoxyvinylglycine mutant enzyme D116A, at 25°C Escherichia coli
4.4.1.13 0.006
-
 aminoethoxyvinylglycine mutant enzyme R58A, at 25°C Escherichia coli
4.4.1.13 0.012
-
 aminoethoxyvinylglycine mutant enzyme R59K, at 25°C Escherichia coli
4.4.1.13 0.04
-
 aminoethoxyvinylglycine mutant enzyme W340F, at 25°C Escherichia coli
4.4.1.13 0.055
-
 aminoethoxyvinylglycine mutant enzyme R58K, at 25°C Escherichia coli
4.4.1.13 9.1
-
 aminoethoxyvinylglycine mutant enzyme R372K, at 25°C Escherichia coli

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
4.4.1.13 0.00134
-
 mutant enzyme R59A, at 25°C Escherichia coli aminoethoxyvinylglycine
4.4.1.13 0.0017
-
 wild type enzyme, at 25°C Escherichia coli aminoethoxyvinylglycine
4.4.1.13 0.00174
-
 mutant enzyme R58A, at 25°C Escherichia coli aminoethoxyvinylglycine
4.4.1.13 0.0026
-
 mutant enzyme D116A, at 25°C Escherichia coli aminoethoxyvinylglycine
4.4.1.13 0.0033
-
 mutant enzyme D116N, at 25°C Escherichia coli aminoethoxyvinylglycine
4.4.1.13 0.0046
-
 mutant enzyme R59K, at 25°C Escherichia coli aminoethoxyvinylglycine
4.4.1.13 0.023
-
 mutant enzyme R58K, at 25°C Escherichia coli aminoethoxyvinylglycine
4.4.1.13 0.043
-
 mutant enzyme W340F, at 25°C Escherichia coli aminoethoxyvinylglycine
4.4.1.13 3.5
-
 mutant enzyme R372K, at 25°C Escherichia coli aminoethoxyvinylglycine

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.4.1.13 0.00009
-
 L-cystathionine mutant enzyme R372A, at 25°C Escherichia coli
4.4.1.13 0.00023
-
 L-cystathionine mutant enzyme R372L, at 25°C Escherichia coli
4.4.1.13 0.385
-
 L-cystathionine mutant enzyme R58K, at 25°C Escherichia coli
4.4.1.13 0.55
-
 L-cystathionine mutant enzyme R372K, at 25°C Escherichia coli
4.4.1.13 1.92
-
 L-cystathionine mutant enzyme R58A, at 25°C Escherichia coli
4.4.1.13 24
-
 L-cystathionine mutant enzyme R59K, at 25°C Escherichia coli
4.4.1.13 45
-
 L-cystathionine mutant enzyme R59A, at 25°C Escherichia coli
4.4.1.13 59
-
 L-cystathionine mutant enzyme W340F, at 25°C Escherichia coli
4.4.1.13 62
-
 L-cystathionine mutant enzyme D116A, at 25°C Escherichia coli
4.4.1.13 69
-
 L-cystathionine mutant enzyme D116N, at 25°C Escherichia coli
4.4.1.13 190
-
 L-cystathionine wild type enzyme, at 25°C Escherichia coli